Journal ArticleDOI
Analysis of serum insulin-like growth factor binding proteins using western blotting: use of the method for titration of the binding proteins and competitive binding studies.
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Titration and competitive binding studies with IGF were performed on the transferred IGF binding proteins, indicating that binding proteins isolated by this technique can be characterized.About:
This article is published in Analytical Biochemistry.The article was published on 1986-04-01. It has received 1170 citations till now. The article focuses on the topics: Electroblotting & Binding protein.read more
Citations
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Journal ArticleDOI
Insulin-Like Growth Factor-Binding Proteins in Serum and Other Biological Fluids: Regulation and Functions
TL;DR: Endocrine Functions of IGFBPs in Serum A. to prevent insulin-like effects B. to increase the half-lives of IGFs C. to exert IGF-independent effects D to control the tra...
Journal ArticleDOI
The insulin-like growth factor-binding protein (IGFBP) superfamily.
TL;DR: The structure similarities between the N-terminal domains of the six IGFBP-rPs and other proteins have been investigated in this article, leading to the concept of the "IGFBP superfamily".
Journal ArticleDOI
Intrauterine Growth Retardation and Postnatal Growth Failure Associated with Deletion of the Insulin-Like Growth Factor I Gene
TL;DR: Insulin-like growth factor I (IGF-I) mediates the majority of the growth-promoting effects of growth hormone (GH) after birth, and fetal tissues express IGF-I from fetal tissues.
Journal ArticleDOI
Liver-derived insulin-like growth factor I (IGF-I) is the principal source of IGF-I in blood but is not required for postnatal body growth in mice
Klara Sjögren,Jun-Li Liu,Kristina Blad,Stanko Skrtic,Olle Vidal,Ville Wallenius,Derek LeRoith,Jan Törnell,Olle Isaksson,John-Olov Jansson,Claes Ohlsson +10 more
TL;DR: It is concluded that postnatal body growth is preserved despite complete absence of IGF-I production by the hepatocytes, suggesting that the liver is the principal source of IGF -I in the blood.
Book ChapterDOI
Insulin-like growth factor binding proteins.
TL;DR: This chapter discusses the insulin-like growth factor (IGF) binding proteins—namely, IGF-I and IGF-II, which are single-chain 7.5-kDa polypeptides that are chemically related to insulin.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Protein blotting: Principles and applications
Jonathan M. Gershoni,G E Palade +1 more
TL;DR: Twenty-eight years ago, Smithies demonstrated that a starch gel could serve as a molecular sieve through which zone electrophoresis of proteins could be carried out and this procedure is now one of the most widely used analytical and preparative tools in cellular and molecular biology.
Journal ArticleDOI
Affinity-labeled plasma somatomedin-C/insulinlike growth factor I binding proteins. Evidence of growth hormone dependence and subunit structure.
J R Wilkins,A J D'Ercole +1 more
TL;DR: The data suggest that the growth hormone (GH)-dependent Sm-C binding protein, represented by the 160,000-mol wt complex, is in some way composed of smaller species, i.e., the 135,000-, 110,000', 80,000, 50,000+, and 28, thousands-24,000 wt complexes.
Journal ArticleDOI
Preferential Measurement of Insulin-Like Growth Factor (IGF) I-Related Peptides in Serum with the Aid of IGF-Binding Proteins (IGF BPs) Produced by Rat Liver in Culture. Estimation of Serum IGF BP Levels*
TL;DR: A protein-binding assay for insulin-like growth factor (IGF) was developed which preferentially measures IGF I-related peptides in serum, which yielded a profile characteristic of IGF I, and by the GH-dependence of the IGF levels measured.
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Insulin-Like Growth Factors and Their Binding Proteins: Biological Actions*
J. I. Jones,David R. Clemmons +1 more
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
Identification and molecular characterization of insulin-like growth factor binding proteins (IGFBP-1, -2, -3, -4, -5 and -6).
Shunichi Shimasaki,Nicholas Ling +1 more