Journal ArticleDOI
Analysis on reassortment of rotavirus NSP1 genes lacking coding region for cysteine-rich zinc finger motif.
TLDR
Reassortment of reassortants with the mutated NSP1 gene and RNA segments from heterologous strains normally replicated in cultured cells showed almost identical growth curve to that of KU, while KU showed a better replication than Aff5–16.Abstract:
Rotavirus clones Aff5–10 and Aff5–16 isolated from a bovine rotavirus strain Aff5 possess NSP1 gene which has a point mutation generating a nonsense codon and a 500 base-deletion, respectively. As a result, the two Aff5 clones encode truncated NSP1 product which lacks cysteine-rich region forming zinc finger motif. In order to analyze reassortment of these mutated NSP1 gene with RNA segments from heterologous strains, we investigated a number of reassortant clones derived from coinfection with either Aff5–10, Aff5–16 or a reference strain Aff5–13 (possessing intact NSP1 gene) and either simian rotavirus SAff11 or human rotavirus KU. In coinfection with SAff11 and Aff5–13, selection rates of Aff5–13 segments in reassortants ranged approximately from 20 to 70% (46% for NSP1 gene). In contrast, in the reassortment between SAff11 and Aff5–10 or between SAff11 and Aff5–16, selection rates of NSP1 gene from Aff5–10 and Aff5–16 were only 1% (one clone) and 0%, respectively. In reassortants from crosses KU × Aff5-clones, selection rate of Aff5–13 NSP1 gene decreased to 15%, while 11 reassortants with Aff5–10 NSP1 gene (31%) and one reassortant with Aff5–16 NSP1 gene (2%) were isolated. Reassortants with Aff5–10 NSP1 possessed a single gene (segment 9 or 11) from KU in the genetic background of Aff5–10. One reassortant clone (cl-55) with Aff5–16 NSP1 gene possessed KU gene segments 3, 4, and 8–11. When single-step growth curves were compared, the reassortant cl-55 showed almost identical growth curve to that of KU, while KU showed a better replication than Aff5–16. These results indicated that although Aff5–10 or Aff5–16 NSP1 gene encoding the truncated NSP1 is selected into reassortants much less efficiently than normal NSP1 gene, the reassortants with the mutated NSP1 gene and RNA segments from heterologous strains normally replicated in cultured cells. Thus, cysteine-rich region of NSP1 was not considered essential for genome segment reassortment with heterologous virus.read more
Citations
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Journal ArticleDOI
Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG).
Jelle Matthijnssens,Max Ciarlet,Sarah M. McDonald,Houssam Attoui,Krisztián Bányai,J. Rodney Brister,Javier Buesa,Mathew D. Esona,Mary K. Estes,Jon R. Gentsch,Miren Iturriza-Gomara,Reimar Johne,Carl D. Kirkwood,Vito Martella,Peter P. C. Mertens,Osamu Nakagomi,Viviana Parreño,Mustafizur Rahman,Franco Maria Ruggeri,Linda J. Saif,Norma Santos,Andrej Steyer,Koki Taniguchi,John T. Patton,Ulrich Desselberger,Marc Van Ranst +25 more
TL;DR: With increasing numbers of complete RV genome sequences becoming available, a standardized RV strain nomenclature system is needed, and the RCWG proposes that individual RV strains are named as follows: RV group/species of origin/country of identification/common name/year of identification /G- and P-type.
Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG) Jelle MatthijnssensMax CiarletSarah M. McDonaldHoussam AttouiKrisztianB anyai • J. Rodney BristerJavier BuesaMathew D. EsonaMary K. EstesJon R. GentschMiren Iturriza-Gomara • Reimar JohneCarl D. KirkwoodVito MartellaPeter P. C. MertensOsamu Nakagomi • Viviana ParrenoMustafizur RahmanFranco M. RuggeriLinda J. SaifNorma Santos • Andrej SteyerKoki TaniguchiJohn T. PattonUlrich DesselbergerMarc Van Ranst
Jelle Matthijnssens,M. Van Ranst,Max Ciarlet,McDonald J. T. Patton,Houssam Attoui,Peter P. C. Mertens,J. R. Brister,J. Buesa,Mathew D. Esona,Jon R. Gentsch,Mary K. Estes,Reimar Johne,Carl D. Kirkwood,Vito Martella +13 more
TL;DR: A nucleotide-sequence-based complete genome classification system was developed for group A rotavirus (RVs) in 2008 as mentioned in this paper, which assigns a specific genotype to each of the 11 genome segments of a par- ticular RV strain according to established nucleotide percent cutoff values.
Journal ArticleDOI
Molecular characterization of human group C rotavirus genes 6, 7 and 9.
TL;DR: Phylogenetic analysis of all the non-structural genes of group A, B and C rotaviruses suggests that these viruses have diverged at a constant rate from a common ancestor.
Journal ArticleDOI
NSP5 phosphorylation regulates the fate of viral mRNA in rotavirus infected cells.
TL;DR: A reassortant strain in the SA11 background that harbours a heterologous segment 11 encoding a variant protein (h-NSP5) is constructed suggesting that NSP5 function in the regulation of the fate of viral positive strand RNA is mediated by phosphorylation.
Journal ArticleDOI
Cell-line-induced mutation of the rotavirus genome alters expression of an IRF3-interacting protein
Karen Kearney,Dayue Chen,Zenobia F. Taraporewala,Patrice Vende,Yasutaka Hoshino,Maria Alejandra Tortorici,Mario Barro,John T. Patton +7 more
TL;DR: It is found that the 3′CS of the gene (g5) encoding NSP1, an antagonist of interferon signaling, undergoes rapid mutation when rhesus rotavirus (RRV) is serially passaged at high multiplicity of infection (MOI) in cells permitting high titer growth.
References
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Journal ArticleDOI
Analysis of reassortment of genome segments in mice mixedly infected with rotaviruses SA11 and RRV.
J L Gombold,R F Ramig +1 more
TL;DR: A few reassortants with specific constellations of SA11 and RRV genome segments were repeatedly isolated from different litters or different animals within single litters, suggesting that these genotypes were independently and specifically selected in vivo.
Journal ArticleDOI
Rotavirus protein NSP3 (NS34) is bound to the 3' end consensus sequence of viral mRNAs in infected cells.
TL;DR: The use of UV cross-linking followed by immunoprecipitation and labeling with T4 polynucleotide kinase allowed us to detect interactions between RNA and nonstructural viral proteins and it is shown that the last 3' nucleotide is cross-linked to the protein and that monomeric and multimeric forms of NSP3 are bound to rotavirus mRNA.
Journal ArticleDOI
Nondefective rotavirus mutants with an NSP1 gene which has a deletion of 500 nucleotides, including a cysteine-rich zinc finger motif-encoding region (nucleotides 156 to 248), or which has a nonsense codon at nucleotides 153-155.
TL;DR: Two nondefective bovine rotavirus mutants (A5-10 and A5-16 clones) which have nonsense mutations in the early portion of the open reading frame of the NSP1 gene replicated well in cultured cells, although the plaque size of A 5-16 was extremely small.
Journal ArticleDOI
Species-specific and interspecies relatedness of NSP1 sequences in human, porcine, bovine, feline, and equine rotavirus strains.
TL;DR: There is a good correlation in most strains between overall genomic property (or genogroup) and NSP1 sequence homology and overall genomic relatedness of strains L26 and YM to various human and animal strains was examined by RNA-RNA hybridization assay.
Journal ArticleDOI
Phenotypes of rotavirus reassortants depend upon the recipient genetic background
TL;DR: The results indicate that the recipient genetic background onto which the genes of a donor rotavirus are reassorted can affect the phenotypes conferred by the presence of the donor segments, and should be interpreted with caution.
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