Journal ArticleDOI
In vivo interactions among rotavirus nonstructural proteins
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TLDR
Immunofluorescence co-localization analysis of virus-infected epithelial cells revealed that the intracellular distribution of proteins that were seen to interact in yeast had patterns of distribution that would allow such intermolecular interactions to occur.Abstract:
The rotavirus genome encodes six nonstructural (NS) proteins, five of which (NSP1, NSP2, NSP3, NSP5, and NSP6) have been suggested to be involved in a variety of events, such as genome replication, regulation of gene expression, and gene assortment. These NS proteins have been found to be associated with replication complexes that are precursors of the viral core, however, little information is available about the intermolecular interactions that may exist among them. Using the yeast two-hybrid system, which allows the detection of protein-protein interactions in vivo, all possible combinations among the rotavirus NS proteins were tested, and several interactions were observed. NSP1 interacted with the other four proteins tested; NSP3 associated with itself; and NSP5 was found to form homodimers and to interact with NSP6. Co-immunoprecipitation of proteins from rotavirus-infected cells, using hyperimmune sera monospecific for the NS proteins, showed the same interactions for NSP1 as those observed in yeast. Immunofluorescence co-localization analysis of virus-infected epithelial cells revealed that the intracellular distribution of proteins that were seen to interact in yeast had patterns of distribution that would allow such intermolecular interactions to occur. These findings should contribute to the understanding of the role these proteins play in different aspects of the virus replication cycle.read more
Citations
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Journal ArticleDOI
Uniformity of rotavirus strain nomenclature proposed by the Rotavirus Classification Working Group (RCWG).
Jelle Matthijnssens,Max Ciarlet,Sarah M. McDonald,Houssam Attoui,Krisztián Bányai,J. Rodney Brister,Javier Buesa,Mathew D. Esona,Mary K. Estes,Jon R. Gentsch,Miren Iturriza-Gomara,Reimar Johne,Carl D. Kirkwood,Vito Martella,Peter P. C. Mertens,Osamu Nakagomi,Viviana Parreño,Mustafizur Rahman,Franco Maria Ruggeri,Linda J. Saif,Norma Santos,Andrej Steyer,Koki Taniguchi,John T. Patton,Ulrich Desselberger,Marc Van Ranst +25 more
TL;DR: With increasing numbers of complete RV genome sequences becoming available, a standardized RV strain nomenclature system is needed, and the RCWG proposes that individual RV strains are named as follows: RV group/species of origin/country of identification/common name/year of identification /G- and P-type.
Journal ArticleDOI
Silencing the morphogenesis of rotavirus.
Tomás López,Minerva Camacho,Margarita Zayas,Rebeca Nájera,Rosana Sanchez,Carlos F. Arias,Susana López +6 more
TL;DR: Results indicate that during a viral infection, NSP4 serves as a receptor for DLPs on the ER membrane and drives the budding of these particles into the ER lumen, while VP7 is required for removing the lipid envelope during the final step of virus morphogenesis.
Journal ArticleDOI
Precise Packaging of the Three Genomic Segments of the Double-Stranded-RNA Bacteriophage φ6
TL;DR: Bacteriophage φ6 has a genome of three segments of double-stranded RNA that is effected by the acquisition, in a serially dependent manner, of the plus strands of the genomic segments into empty procapsids.
Journal ArticleDOI
Characterization of Rotavirus Cell Entry
TL;DR: Interestingly, cells treated with methyl-β-cyclodextrin, a drug that sequesters cholesterol from membranes, and cells expressing a dominant-negative mutant of the large GTPase dynamin were not infected by rotavirus, indicating that cholesterol and dynamin play a role in the entry of rotaviruses.
Journal ArticleDOI
Interferon Regulatory Factor 3 Is a Cellular Partner of Rotavirus NSP1
TL;DR: It is predicted that a role for NSP1 in rotavirus-infected cells is to inhibit activation of IRF-3 and diminish the cellular interferon response.
References
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Book
Experiments in molecular genetics
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Journal ArticleDOI
A novel genetic system to detect protein-protein interactions.
Stanley Fields,Ok-kyu Song +1 more
TL;DR: A novel genetic system to study protein-protein interactions between two proteins by taking advantage of the properties of the GAL4 protein of the yeast Saccharomyces cerevisiae, which may be applicable as a general method to identify proteins that interact with a known protein by the use of a simple galactose selection.
Journal ArticleDOI
The retinoblastoma protein associates with the protein phosphatase type 1 catalytic subunit.
Tim Durfee,Kathleen Becherer,Phang Lang Chen,Shiou-Hwei Yeh,Yanzhu Yang,April E. Kilburn,Wen-Hwa Lee,Stephen J. Elledge +7 more
TL;DR: An improved version of the yeast two-hybrid system is developed and used to isolate human cDNAs encoding proteins able to bind p110RB to demonstrate that PP-1 alpha isoforms preferentially bind the hypophosphorylated form of p110 RB.
Journal ArticleDOI
The two-hybrid system: an assay for protein-protein interactions
Stanley Fields,Rolf Sternglanz +1 more
TL;DR: The two-hybrid system is a yeast-based genetic assay for detecting protein-protein interactions that has been developed to clone genes that encode DNA-binding proteins, to identify peptides that bind to a protein and, potentially, to screen for drugs.
Journal ArticleDOI
A new class of yeast transcriptional activators.
Jun Ma,Mark Ptashne +1 more
TL;DR: Yeast transcriptional activators encoded by E. coli genomic DNA fragments fused to the coding sequence of the DNA-binding portion of GAL4 and a fusion protein that contains no yeast protein sequence but activates transcription in yeast are described.