Journal ArticleDOI
Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli.
Reads0
Chats0
TLDR
The combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually.Abstract:
The antibacterial activity and acting mechanism of silver nanoparticles (SNPs) on Escherichia coli ATCC 8739 were investigated in this study by analyzing the growth, permeability, and morphology of the bacterial cells following treatment with SNPs. The experimental results indicated 10 microg/ml SNPs could completely inhibit the growth of 10(7) cfu/ml E. coli cells in liquid Mueller-Hinton medium. Meanwhile, SNPs resulted in the leakage of reducing sugars and proteins and induced the respiratory chain dehydrogenases into inactive state, suggesting that SNPs were able to destroy the permeability of the bacterial membranes. When the cells of E. coli were exposed to 50 microg/ml SNPs, many pits and gaps were observed in bacterial cells by transmission electron microscopy and scanning electron microscopy, and the cell membrane was fragmentary, indicating the bacterial cells were damaged severely. After being exposed to 10 microg/ml SNPs, the membrane vesicles were dissolved and dispersed, and their membrane components became disorganized and scattered from their original ordered and close arrangement based on TEM observation. In conclusion, the combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually.read more
Citations
More filters
Journal ArticleDOI
Bacterial Cellulose (BC) and BC Composites: Production and Properties
Tatiana G. Volova,Svetlana V. Prudnikova,Evgeniy G. Kiselev,Ivan V. Nemtsev,Alexander D. Vasiliev,Andrey Kuzmin,Ekaterina I. Shishatskaya +6 more
TL;DR: The disc-diffusion method showed pronounced antibacterial activity of BC composites against E. coli AT CC 25922 and S. aureus ATCC 25923 and the highest productivity of the BC synthesis process was obtained with the growth of bacteria on molasses and glycerol.
Journal ArticleDOI
Hollow selenium nanoparticles from potato extract and investigation of its biological properties and developmental toxicity in zebrafish embryos.
TL;DR: The results of this study indicate that potato extract reduces the toxicity of hSeNPs and lower concentrations of h SeNPs could be used for various biomedical applications in near future.
Journal ArticleDOI
Ag-Doped and Antibiotic-Loaded Hexagonal Boron Nitride Nanoparticles as Promising Carriers to Fight Different Pathogens.
Kristina Yu. Gudz,Liubov Yu. Antipina,Elizaveta S. Permyakova,Andrey M. Kovalskii,Anton S. Konopatsky,Svetlana Yu. Filippovich,I.A. Dyatlov,Pavel V. Slukin,Sergei G. Ignatov,Dmitry V. Shtansky +9 more
TL;DR: In this article, the authors demonstrate that Antibacterial components-conjugated nanoparticles (NPs) and antibiotic-loaded BN and Ag/BN nanoconjugates are promising carriers to fight bacterial and fungal infections.
Journal ArticleDOI
Spectroscopic characterization and photodynamic antimicrobial chemotherapy of phthalocyanine-silver triangular nanoprism conjugates when supported on asymmetric polymer membranes.
TL;DR: Conjugation of nanoparticles increased triplet state and singlet oxygen quantum yields of the phthalocyanines as well as enhancing photodynamic antimicrobial chemotherapy (PACT) activity against bacteria (S. aureus).
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria
Ivan Sondi,Branka Salopek-Sondi +1 more
TL;DR: These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials.
Journal ArticleDOI
Silver nanoparticles as a new generation of antimicrobials.
TL;DR: Silver nanoparticles have emerged up with diverse medical applications ranging from silver based dressings, silver coated medicinal devices, such as nanogels, nanolotions, etc, due to its capability of modulating metals into their nanosize.
Journal ArticleDOI
Proteomic analysis of the mode of antibacterial action of silver nanoparticles
Chun-Nam Lok,Chi-Ming Ho,Rong Chen,Qing-Yu He,Wing-Yiu Yu,Hongzhe Sun,Paul K.H. Tam,Jen-Fu Chiu,Chi-Ming Che +8 more
TL;DR: Silver nanoparticles (nano-Ag) are potent and broad-spectrum antimicrobial agents and appear to be an efficient physicochemical system conferring antimicrobial silver activities.
Related Papers (5)
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria
Ivan Sondi,Branka Salopek-Sondi +1 more