Journal ArticleDOI
Antibacterial activity and mechanism of silver nanoparticles on Escherichia coli.
Reads0
Chats0
TLDR
The combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually.Abstract:
The antibacterial activity and acting mechanism of silver nanoparticles (SNPs) on Escherichia coli ATCC 8739 were investigated in this study by analyzing the growth, permeability, and morphology of the bacterial cells following treatment with SNPs. The experimental results indicated 10 microg/ml SNPs could completely inhibit the growth of 10(7) cfu/ml E. coli cells in liquid Mueller-Hinton medium. Meanwhile, SNPs resulted in the leakage of reducing sugars and proteins and induced the respiratory chain dehydrogenases into inactive state, suggesting that SNPs were able to destroy the permeability of the bacterial membranes. When the cells of E. coli were exposed to 50 microg/ml SNPs, many pits and gaps were observed in bacterial cells by transmission electron microscopy and scanning electron microscopy, and the cell membrane was fragmentary, indicating the bacterial cells were damaged severely. After being exposed to 10 microg/ml SNPs, the membrane vesicles were dissolved and dispersed, and their membrane components became disorganized and scattered from their original ordered and close arrangement based on TEM observation. In conclusion, the combined results suggested that SNPs may damage the structure of bacterial cell membrane and depress the activity of some membranous enzymes, which cause E. coli bacteria to die eventually.read more
Citations
More filters
Journal ArticleDOI
Bactericidal, quorum quenching and anti-biofilm nanofactories: a new niche for nanotechnologists.
Brahma N. Singh,Prateeksha,Dalip K. Upreti,Braj Raj Singh,Tom Defoirdt,Vijai Kumar Gupta,Ana O. de Souza,Harikesh Bahadur Singh,João C.M. Barreira,Isabel C.F.R. Ferreira,Khabat Vahabi +10 more
TL;DR: This communication summarizes emerging nano-antimicrobial therapies in treating bacterial infections, particularly using antibacterial, quorum quenching, and anti-biofilm nanomaterials as new approaches to tackle the current challenges in combating infectious diseases.
Journal Article
Applications of Silver nanoparticles in diverse sectors
TL;DR: In this article, a review article summarizes the applications of silver nanoparticles for diverse sectors and concludes that over 23% of all nanotechnology based products, diagnostic and therapeutic applications implanted with Silver nanoparticles (e.g., antifungal, antibacterial, antiviral effect, employed in textile fabrics and added into cosmetic products as antiseptic to overcome skin problems).
Journal ArticleDOI
Low Temperature Deposition of Antibacterially Active Silicon Oxide Layers Containing Silver Nanoparticles, Prepared by Atmospheric Pressure Plasma Chemical Vapor Deposition
Oliver Beier,Andreas Pfuch,Kerstin Horn,Jürgen Weisser,Matthias Schnabelrauch,Arnd Schimanski +5 more
Journal ArticleDOI
Environmental impact of biogenic silver nanoparticles in soil and aquatic organisms
TL;DR: Investigation of the effects of different concentrations of mangrove fungus Aspergillus tubingensis AgNPs on the aerobic heterotrophs soil microorganisms, rice seeds and zebrafish found them to be less harmful for soil microbiota compared to AgNO3.
Journal ArticleDOI
Tuber extract of Arisaema flavum eco-benignly and effectively synthesize silver nanoparticles: Photocatalytic and antibacterial response against multidrug resistant engineered E. coli QH4
Aziz Ur Rahman,Arif Ullah Khan,Qipeng Yuan,Yun Wei,Aftab Ahmad,Sadeeq Ullah,Zia Ul Haq Khan,Saira Shams,Muhammad Tariq,Waqas Ahmad +9 more
TL;DR: The antibacterial properties of the AgNPs prepared with various extracts were tested against multi-drug resistant bacteria and showed significant antibacterial activity against all the multidrug resistant bacterial strains and especially multidrog resistant engineered E.coli QH4.Coli.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
Use of Dinitrosalicylic Acid Reagent for Determination of Reducing Sugar
Journal ArticleDOI
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria
Ivan Sondi,Branka Salopek-Sondi +1 more
TL;DR: These nontoxic nanomaterials, which can be prepared in a simple and cost-effective manner, may be suitable for the formulation of new types of bactericidal materials.
Journal ArticleDOI
Silver nanoparticles as a new generation of antimicrobials.
TL;DR: Silver nanoparticles have emerged up with diverse medical applications ranging from silver based dressings, silver coated medicinal devices, such as nanogels, nanolotions, etc, due to its capability of modulating metals into their nanosize.
Journal ArticleDOI
Proteomic analysis of the mode of antibacterial action of silver nanoparticles
Chun-Nam Lok,Chi-Ming Ho,Rong Chen,Qing-Yu He,Wing-Yiu Yu,Hongzhe Sun,Paul K.H. Tam,Jen-Fu Chiu,Chi-Ming Che +8 more
TL;DR: Silver nanoparticles (nano-Ag) are potent and broad-spectrum antimicrobial agents and appear to be an efficient physicochemical system conferring antimicrobial silver activities.
Related Papers (5)
Silver nanoparticles as antimicrobial agent: a case study on E. coli as a model for Gram-negative bacteria
Ivan Sondi,Branka Salopek-Sondi +1 more