Atomically unveiling the structure-activity relationship of biomacromolecule-metal-organic frameworks symbiotic crystal
Linjing Tong,Siming Huang,Yujian Shen,Suya Liu,Xiaomin Ma,Fang Zhu,Guosheng Chen,Gangfeng Ouyang +7 more
TLDR
In this paper , the atomic-level structure of biomacromolecules-metal-organic frameworks (BMOFs) was identified using differential phase contrast-scanning transmission electron microscopy, cryo-electron microscopy and x-ray absorption fine structure techniques.Abstract:
Crystallization of biomacromolecules-metal-organic frameworks (BMOFs) allows for orderly assemble of symbiotic hybrids with desirable biological and chemical functions in one voxel. The structure-activity relationship of this symbiotic crystal, however, is still blurred. Here, we directly identify the atomic-level structure of BMOFs, using the integrated differential phase contrast-scanning transmission electron microscopy, cryo-electron microscopy and x-ray absorption fine structure techniques. We discover an obvious difference in the nanoarchitecture of BMOFs under different crystallization pathways that was previously not seen. In addition, we find the nanoarchitecture significantly affects the bioactivity of the BMOFs. This work gives an important insight into the structure-activity relationship of BMOFs synthesized in different scenarios, and may act as a guide to engineer next-generation materials with excellent biological and chemical functions. read more
Citations
More filters
Journal ArticleDOI
Confining enzymes in porous organic frameworks: from synthetic strategy and characterization to healthcare applications.
TL;DR: The state-of-the-art strategies for the structural immobilization of enzymes using the well-explored MOFs and burgeoning COFs and HOFs as scaffolds are described, with special emphasis on how these porous framework-confined technologies can provide a favorable microenvironment for mimicking natural biocatalysis.
Journal ArticleDOI
Hydrogen-bonded organic framework biomimetic entrapment allowing non-native biocatalytic activity in enzyme
TL;DR: In this article , an exogenous hydrogen-bonded organic framework was designed to modulate the conformation of cytochrome c, and hence allow non-native bioactivity for the enzyme.
Journal ArticleDOI
Ultrathin Metal-Organic Framework Nanosheets Exhibiting Exceptional Catalytic Activity.
Rong-Jia Wei,Peihong You,Haiyan Duan,Mo-Wen Xie,Ri-Qin Xia,Xu Chen,Xiaoxu Zhao,Guo-Hong Ning,Andrew I. Cooper,Dan Li +9 more
TL;DR: In this article , the synthesis of metal-organic framework nanosheets (MONs) via interfacial polymerization is reported, which involves three active monomers for simultaneous polycondensation and polycoordination taking place in a confined interface, and the well-defined lamellar structure of the MOF films allowed feasible and scalable exfoliation to produce freestanding 2D MONs with high aspect ratio up to 2000:1 and ultrathin thickness (∼1.7 nm).
Journal ArticleDOI
Enhanced Activity of Enzyme Immobilized on Hydrophobic ZIF-8 Modified by Ni2+ Ions.
TL;DR: In this paper , a facile synthesis approach was developed to immobilize pepsin (PEP) on the ZIF-8 carrier by using Ni2+ ions as anchor, which achieved remarkable OER performance with an ultralow overpotential of only 127 mV at 10 mA•cm-2.
Journal ArticleDOI
Enzymes-Encapsulated Defective Metal-Organic Framework Hydrogel Coupling with a Smartphone for a Portable Glucose Biosensor.
Ningyi Zhong,Rui Gao,Yujian Shen,Xiaoxue Kou,Jiayi Wu,Siming Huang,Guosheng Chen,Gangfeng Ouyang +7 more
TL;DR: A robust and sensitive hybrid biosensor by means of co-encapsulating enzymes into a defective metal-organic framework (MOF), followed by a double-crosslinked alginate gelatinization, enabling the colorimetric biosensing of glucose on a miniaturized MOF hydrogel when coupling with a smartphone.
References
More filters
Journal ArticleDOI
A rapid and sensitive method for the quantitation of microgram quantities of protein utilizing the principle of protein-dye binding
TL;DR: This assay is very reproducible and rapid with the dye binding process virtually complete in approximately 2 min with good color stability for 1 hr with little or no interference from cations such as sodium or potassium nor from carbohydrates such as sucrose.
Journal ArticleDOI
ATHENA, ARTEMIS, HEPHAESTUS: data analysis for X-ray absorption spectroscopy using IFEFFIT.
Bruce Ravel,Matthew Newville +1 more
TL;DR: A software package for the analysis of X-ray absorption spectroscopy (XAS) data is presented, based on the IFEFFIT library of numerical and XAS algorithms and is written in the Perl programming language using the Perl/Tk graphics toolkit.
Journal ArticleDOI
Biomimetic mineralization of metal-organic frameworks as protective coatings for biomacromolecules.
Kang Liang,Raffaele Ricco,Cara M. Doherty,Mark J. Styles,Stephen Bell,Nigel Kirby,Stephen T. Mudie,David N. Haylock,Anita J. Hill,Christian J. Doonan,Paolo Falcaro +10 more
TL;DR: It is shown that proteins, enzymes and DNA rapidly induce the formation of protective metal-organic framework coatings under physiological conditions by concentrating the framework building blocks and facilitating crystallization around the biomacromolecules.
Journal ArticleDOI
One-pot Synthesis of Metal-Organic Frameworks with Encapsulated Target Molecules and Their Applications for Controlled Drug Delivery.
TL;DR: It is demonstrated that large drug and dye molecules can be encapsulated in zeolitic imidazolate framework (ZIF) crystals, and it is shown that ZIF-8 crystals loaded with the anticancer drug doxorubicin (DOX) are efficient drug delivery vehicles in cancer therapy using pH-responsive release.
Journal ArticleDOI
Atomic structure of sensitive battery materials and interfaces revealed by cryo–electron microscopy
Yuzhang Li,Yanbin Li,Allen Pei,Kai Yan,Yongming Sun,Chun-Lan Wu,Lydia-Marie Joubert,Richard Chin,Ai Leen Koh,Yi Yu,John Perrino,Benjamin Butz,Benjamin Butz,Steven Chu,Yi Cui,Yi Cui +15 more
TL;DR: Cryo–electron microscopy can preserve reactive metals and thus reveal the atomic structure of a lithium metal dendrite and atomically resolve individual lithium metal atoms and their interface with the solid electrolyte interphase (SEI).