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Bacterial Acetone Carboxylase Is a Manganese-dependent Metalloenzyme

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TLDR
It is demonstrated that manganese is essential for acetone carboxylation and a role for manganes in nucleotide binding and activation is suggested and suggested.
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This article is published in Journal of Biological Chemistry.The article was published on 2004-11-05 and is currently open access. It has received 36 citations till now. The article focuses on the topics: Acetone carboxylase & Manganese.

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Carboxylases in Natural and Synthetic Microbial Pathways

TL;DR: The physiological roles of carboxylases in different microbial pathways that range from autotrophy, carbon assimilation, and anaplerosis to biosynthetic and redox-balancing functions are discussed.
Journal ArticleDOI

Functional proteomic view of metabolic regulation in "Aromatoleum aromaticum" strain EbN1.

TL;DR: Comparing all identified regulated and nonregulated proteins provided first insights into regulatory hierarchies of special degradation pathways versus general metabolism in strain EbN1, and strong evidence was obtained for thus far unpredicted degradation pathways of three hitherto unknown substrates.
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Metal centers in the anaerobic microbial metabolism of CO and CO2

TL;DR: Studies of CO and CO(2) enzymology are of practical significance, and offer fundamental insights into important biochemical reactions involving metallocenters that act as nucleophiles to form organometallic intermediates and catalyze C-C and C-S bond formations.
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ATP-Dependent Carboxylation of Acetophenone by a Novel Type of Carboxylase

TL;DR: Observations indicate that both substrates may be phosphorylated, which is consistent with isotope exchange activity observed with deuterated acetophenone and inhibition by carbamoylph phosphate, a structural analogue of carboxyphosphate.
References
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Journal ArticleDOI

Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4

TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article

Cleavage of structural proteins during the assemble of the head of bacterio-phage T4

U. K. Laemmli
- 01 Jan 1970 - 
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
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The Mechanism of Biotin-Dependent Enzymes

TL;DR: The mechanism of Biotin Carboxylation, and some Suggested Mechanisms and Efforts to Discriminate amongst Them, are described.
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Identification of ethanol-inducible P-450 isozyme 3a as the acetone and acetol monooxygenase of rabbit microsomes.

TL;DR: The results suggest that isozyme 3a in rabbit and an immunochemically homologous enzyme in rat are responsible for acet one and acetol hydroxylation, the initial steps in the proposed gluconeogenic pathways for acetone.
Journal ArticleDOI

Paramagnetic Resonance of Mn2+ in Glasses and Compounds of the Lithium Borate System

TL;DR: In this paper, the paramagnetic resonance spectra of Mn2+ ions in glasses and compounds of the lithium borate system have been investigated at 9.1 and 35 GHz, and it is concluded that many of the sites in the glasses are probably distorted versions of the site occupied in the Li2O·4B2O3 compound.
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