Journal ArticleDOI
Bio-assay Guided Isolation of α-Glucosidase Inhibitory Constituents from Hibiscus Mutabilis Leaves
TLDR
The identification of α-glucosidase inhibition activity in H. mutabilis supports further investigations into the possible use of the plant for the management of diabetes and the HPLC method validated for these extracts will be useful in future research with the plant.Abstract:
Introduction
The increasing demand for natural-product-based medicines and health-care products for the management of diabetes encouraged investigation of this commonly available Indian plant.
Objectives
To establish the anti-diabetic (α-glucosidase inhibitory) activity of H. mutabilis leaf extract, isolate and identify the constituents responsible for the activity, and validate a HPLC method for quantification of the active constituents for standardisation of the extract.
Material and methods
The methanolic extract of leaves was partitioned between water, n-butanol and ethyl acetate. Bio-assay guided fractionation, based on inhibition of α-glucosidase, allowed isolation and identification of the active components. The active components were quantified using RP-HPLC-DAD validated for linearity, limit of detection, limit of quantification, precision, accuracy and robustness for this plant extract and the partitioned fractions.
Results
Ferulic acid and caffeic acid were identified as the α-glucosidase inhibitors present in H. mutabilis. They were partitioned into an ethyl acetate fraction. The HPLC-DAD calibration curve showed good linearity (r2 > 0.99). For the recovery studies the %RSD was less than 2%. The interday and intraday variations were found to be less than 4% RSD for retention time and response.
Conclusion
The identification of α-glucosidase inhibition activity in H. mutabilis supports further investigations into the possible use of the plant for the management of diabetes. The HPLC method validated for these extracts will be useful in future research with the plant. Copyright © 2011 John Wiley & Sons, Ltd.read more
Citations
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Journal ArticleDOI
Immobilized magnetic beads based multi-target affinity selection coupled with high performance liquid chromatography-mass spectrometry for screening anti-diabetic compounds from a Chinese medicine "Tang-Zhi-Qing".
TL;DR: The proposed approach for screening bioactive compounds from botanical drug using multiple target-immobilized magnetic beads coupled with high performance liquid chromatography-mass spectrometry is suggested to be efficient in screening compounds with multiple activities from extracts of botanical drugs.
Journal ArticleDOI
Metabolomics analysis and biological investigation of three Malvaceae plants
Omnia Hesham Abdelhafez,Eman M. Othman,John Refaat Fahim,Samar Yehia Desoukey,Sheila M. Pimentel-Elardo,Justin R. Nodwell,Tanja Schirmeister,Ahmed F. Tawfike,Ahmed F. Tawfike,Usama Ramadan Abdelmohsen +9 more
TL;DR: This study highlighted the use of the established procedure in exploring the metabolomes of three Malvaceae plants, which could be helpful for chemotaxonomic and authentication purposes, and might expand the basis for their future phytochemical analysis.
Journal ArticleDOI
α-Glucosidase inhibitory terpenoids from Potentilla fulgens and their quantitative estimation by validated HPLC method
TL;DR: In this paper, the A-ring contracted triterpenes with α-glucosidase inhibitory activity were identified from Potentilla fulgens, which is used extensively in traditional system of medicine and as a food additive for the treatment of diabetes mellitus and other ailments.
Journal ArticleDOI
α-Glucosidase and α-amylase inhibitory constituent of Carex baccans: Bio-assay guided isolation and quantification by validated RP-HPLC–DAD
TL;DR: The identification of α-glucosidase and α-amylase inhibitory activity in C. baccans supports the possible use of the plant as functional food for the management of diabetes and the validated HPLC method for the study of plant extracts will be useful in future research.
Journal ArticleDOI
In vitro antioxidant assessment, screening of enzyme inhibitory activities of methanol and water extracts and gene expression in Hypericum lydium.
Nuraniye Eruygur,Esra Ucar,Huseyin Askin Akpulat,Keyhan Shahsavari,Seyed Mehdi Safavi,Danial Kahrizi,Danial Kahrizi +6 more
TL;DR: The purpose of this study is to study the active molecules such as antioxidant, antimicrobial, antidiabetic, enzymes and genes in the plant, which is the first to be reported.
References
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Journal ArticleDOI
Glucosidase inhibition. A new approach to the treatment of diabetes, obesity, and hyperlipoproteinaemia
Journal ArticleDOI
Screening and Structural Characterization of α-Glucosidase Inhibitors from Hawthorn Leaf Flavonoids Extract by Ultrafiltration LC-DAD-MSn and SORI-CID FTICR MS
TL;DR: Four compounds were identified as α-glucosidase inhibitors in the HLFE, and their structures were confirmed to be quercetin-3-O-rha- (1-4)-glc-rha and C-glycosylflavones by high-resolution sustained off resonance irradiation collision-induced dissociation (SORI-CID) data obtained by Fourier transform ion cyclotron resonance mass spectrometry (FTICR MS).
Journal ArticleDOI
A series of cinnamic acid derivatives and their inhibitory activity on intestinal alpha-glucosidase
TL;DR: This study investigated the inhibitory activity of cinnamic acid derivatives against rat intestinal α-glucosidase and porcine pancreatic α-amylase in vitro and found that intestinal maltase was inhibited by caffeic acid, ferulic acid, and isoferulic acid in a mixed-inhibition manner.
Journal ArticleDOI
Universally occurring phenylpropanoid and species-specific indolic metabolites in infected and uninfected Arabidopsis thaliana roots and leaves
TL;DR: Comparison of these results with analogous data on a variety of different plant species suggests a remarkable structural uniformity among the majority of constitutive as well as infection-induced, aromatic cell wall-bound compounds throughout the entire plant kingdom.
Journal ArticleDOI
α-Glucosidase inhibitors from Devil tree (Alstonia scholaris)
TL;DR: Potent α-glucosidase inhibitory activity was found in aqueous methanol extract of dried Devil tree ( Alstonia scholaris ) leaves, and the structures of these isolated compounds were found to be quercetin 3- O -β- d -xylopyranosyl and (−)-lyoniresinol.