Book ChapterDOI
Bioaffinity Based Immobilization of Enzymes
TLDR
Bioaffinity based immobilization procedures usually yield preparations exhibiting high catalytic activity and improved stability against denaturation, facilitating the reuse of support matrix, orient the enzymes favourably and offer the possibility of enzyme immobilization directly from partially pure enzyme preparations or even cell lysates.Abstract:
Procedures that utilize the affinities of biomolecules and ligands for the immobilization of enzymes are gaining increasing acceptance in the construction of sensitive enzyme-based analytical devices as well as for other applications. The strong affinity of polyclonal/monoclonal antibodies for specific enzymes and those of lectins for glycoenzymes bearing appropriate oligosaccharides have been generally employed for the purpose. Potential of affinity pairs like cellulose-cellulose binding domain bearing enzymes and immobilized metal ion-surface histidine bearing enzymes has also been recognised. The bioaffinity based immobilization procedures usually yield preparations exhibiting high catalytic activity and improved stability against denaturation. Bioaffinity based immobilizations are usually reversible facilitating the reuse of support matrix, orient the enzymes favourably and offer the possibility of enzyme immobilization directly from partially pure enzyme preparations or even cell lysates. Enzyme lacking innate ability to bind to various affinity supports can be made to bind to them by chemically or genetically linking the enzymes with appropriate polypeptides/domains like the cellulose binding domain, protein A, histidine-rich peptides, single chain antibodies, etc.read more
Citations
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Journal ArticleDOI
Potential of Different Enzyme Immobilization Strategies to Improve Enzyme Performance
TL;DR: The advantages and disadvantages of the different existing immobilization strategies to solve the different aforementioned enzyme limitations are given and some advice to select the optimal strategy for each particular enzyme and process is given.
Journal ArticleDOI
Strategies for the one-step immobilization–purification of enzymes as industrial biocatalysts
Oveimar Barbosa,Claudia Ortiz,Ángel Berenguer-Murcia,Rodrigo Torres,Rafael C. Rodrigues,Roberto Fernandez-Lafuente +5 more
TL;DR: The development of tailor-made heterofunctional supports as a tool to immobilize-stabilize-purify some proteins will be discussed in deep, using low concentration of adsorbent groups and a dense layer of groups able to give an intense multipoint covalent attachment.
Journal ArticleDOI
Carbohydrate Binding Modules: Biochemical Properties and Novel Applications
TL;DR: Based on their properties, CBMs are grouped into 43 families that display substantial variation in substrate specificity, along with other properties that make them a gold mine for biotechnologists who seek natural molecular "Velcro" for diverse and unusual applications.
Journal ArticleDOI
Current and prospective applications of metal ion–protein binding
TL;DR: Applications in protein processing are described and variations of immobilized metal ion affinity chromatography are described as well as other metal affinity-based technologies.
Journal ArticleDOI
Selective immobilization of proteins to self-assembled monolayers presenting active site-directed capture ligands
TL;DR: This capture ligand immobilization method combines the advantages that the immobilization reaction is highly selective for the intended protein, the tether is covalent and, hence, stable, and the method avoids the need for synthetic modification and rigorous purification of proteins before immobilization.
References
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Journal ArticleDOI
Principles of protein-protein interactions
Susan Jones,Janet M. Thornton +1 more
TL;DR: This review examines protein complexes in the Brookhaven Protein Databank to gain a better understanding of the principles governing the interactions involved in protein-protein recognition.
Journal ArticleDOI
Metal chelate affinity chromatography, a new approach to protein fractionation
TL;DR: A highly flexible method based on affinities which can be used in a more selective fashion by modern chromatographic techniques is described here.
Book ChapterDOI
Protein denaturation. C. Theoretical models for the mechanism of denaturation.
TL;DR: This chapter reviews theoretical models that might be constructed and equations that may be derived from them to understand the process of protein denaturation and finds that they can be predicted semiquantitatively.
Book
Monoclonal Antibodies: Principles and Practice
TL;DR: The present volume has been comprehensively updated to cover recent rapid advances, particularly with respect to the applications of molecular biology, the use of antibodies in closing and heterologous expression of genes, immunohistology and phage display libraries.
Journal ArticleDOI
New metal chelate adsorbent selective for proteins and peptides containing neighbouring histidine residues.
TL;DR: A novel nitrilotriacetic acid adsorbent has been prepared for metal chelate affinity chromatography and has been found to chelate Cu2+ and Ni2+ strongly and to be superior to the known iminodiacetic acid Adsorbent.