Journal ArticleDOI
Biocatalytic microreactor incorporating HRP anchored on micro-/nano-lithographic patterns for flow oxidation of phenols
Madalina Tudorache,Diana Mahalu,Cristian M. Teodorescu,Razvan C. Stan,Camelia Bala,Vasile I. Parvulescu +5 more
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TLDR
In this paper, a highly durable and versatile micro-scale biocatalytic reactor has been designed and investigated in the heterogeneous enzyme-catalytic oxidation of phenols.Abstract:
A highly durable and versatile micro scale biocatalytic reactor has been designed and investigated in the heterogeneous enzyme-catalytic oxidation of phenols. It consisted of optical or electron beam lithographic gold deposited layer with different thickness from nano- to micro-scale on a semiconductor surface. Horseradish peroxidase (HRP) enzyme was immobilized on the gold-surface using two different approaches (i.e., physical adsorption and self-assembled monolayer). Characterization of those HRP-gold surfaces was made using AFM (atomic force microscopy) coupled with ellipsometry measurements, SEM (scanning electron microscopy), STM (scanning tunneling microscopy) and spectrophotometric technique. The catalytic performances of the developed structures were investigated after their incorporation in microreactors using a recycling-flow system dedicated to phenols oxidation. In order to optimize the efficiency of the oxidation process it was checked the effect of the successively addition of the reagent, the influence of the reaction time, the stability and the reuse of the biocatalytic micro-system. Maximum phenol conversion was of 35.1%. In addition to phenol the system has also been investigated in oxidation of other phenolic compounds such as cathecol, hydroquinone, rezorcine, β-naphtol, o/p-aminophenol and 4-metoxyphenol.read more
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Journal ArticleDOI
Liquid phase oxidation chemistry in continuous-flow microreactors
Hannes P. L. Gemoets,Yuanhai Su,Minjing Shang,Volker Hessel,Rafael Luque,Timothy Noël,Timothy Noël +6 more
TL;DR: An up-to-date overview of both technological and chemical aspects of liquid phase oxidation chemistry in continuous-flow microreactors is given, including the use of oxygen, hydrogen peroxide, ozone and other oxidants in flow.
Journal ArticleDOI
Continuous flow biocatalysis.
TL;DR: This Review explores continuous flow biocatalysts with emphasis on new technology, enzymes, whole cells, co-factor recycling, and immobilization methods for the synthesis of pharmaceuticals, value-added chemicals, and materials.
Journal ArticleDOI
Enzyme-Immobilized Microfluidic Process Reactors
TL;DR: This review summarizes the recent advances of microchannel reaction technologies especially for enzyme immobilized microreactors, and discusses the manufacturing process of microreaction devices and the advantages of micro Reactors compared to conventional reaction devices.
Journal ArticleDOI
Microfluidic devices: useful tools for bioprocess intensification.
TL;DR: The goal of the present work is to provide an updated overview of the developments centered on the use of microfluidic devices for process intensification in biotechnology, with particular focus on different designs, configurations and modes of operation of microreactors.
Journal ArticleDOI
Microfluidic reactors with immobilized enzymes—Characterization, dividing, perspectives
TL;DR: In this article, a review of various types of microfluidic IMERs is presented, focusing on techniques of their preparation including immobilization strategies and technical solutions connected with their applications using both capillary and chip format.
References
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TL;DR: Under the new conditions there is direct proportionality between absorbance at 650 nm and weight of protein within the range 15–110 μg.
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Potential applications of oxidative enzymes and phenoloxidase-like compounds in wastewater and soil treatment: a review
Nelson Durán,Elisa Esposito +1 more
TL;DR: Immobilization of oxidative enzymes on porous ceramic supports or resins did not adversely affect their stability and showed a good potential for degradation of environment persistent aromatics.
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Self-assembled monolayers as a tunable platform for biosensor applications.
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Polymer Synthesis by In Vitro Enzyme Catalysis
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