Showing papers in "Biosensors and Bioelectronics in 2002"

TL;DR: In the present review an attempt has been made to describe the salient features of conducting polymers and their wide applications in health care, food industries, environmental monitoring etc.

TL;DR: It is demonstrated with suitable examples that monolayer design plays a key role in controlling the performance of these SAM based biosensors, irrespective of the immobilisation strategy and sensing mechanism.

TL;DR: A new physical effect 'missing mass' of the sample in liquid phase measurements is predicted and a fundamental result of the theory is the analog of Sauerbrey relation for layered viscous/viscoelastic medium which can be exploited for the correct physical interpretation of QCM experimental data in biofluids.

TL;DR: The present study reports on the use of p(2-hydroxyethyl methacrylate) (pHEMA) in which polypyrrole and various oxidoreductase enzymes were physically entrapped to function as a viable matrix for the construction of clinically important amperometric biosensors.

TL;DR: The first use of the silicone elastomer, poly(dimethylsiloxane) (PDMS), for patch clamp electrodes is described, indicating the potential for high-throughput patch clamp recording with a planar array of PDMS electrodes.

TL;DR: A novel glutaminase with an activity optimum in the neutral pH range direct and simultaneous monitoring of glutamine together with glucose, lactate, and glutamate could be performed.

TL;DR: The enzyme horseradish peroxidase has been entrapped in situ by electropolymerization of pyrrole onto a platinum electrode and the biosensor retained more than 90% of its original activity after 35 days of use.

TL;DR: A fiber optic surface plasmon resonance (SPR) biosensor for detection of Staphylococcal enterotoxin B is reported and is demonstrated to be able to detect ng/ml concentrations of SEB in less than 10 min.

TL;DR: In this paper, a two-channel surface plasmon resonance (SPR) biosensors were used for real-time monitoring of Staphylococcus aureus enterotoxin B (SEB).

TL;DR: There is an excellent scope for the application of biosensors in the seafood industry including the rapidly expanding aquaculture operations for fast assessment of quality.

TL;DR: The potential for early detection of microbial contaminants in urine samples using electronic nose technology for the first time is shown and will have implications for the development of rapid systems for use in clinical practice.

TL;DR: A new glucose sensor that utilized an outer permselective membrane made of amphiphobic polyurethane which allows glucose passage through hydrophilic segments was designed that was more predictable in vitro as evidenced by a much lower variation of glucose sensitivity than the original protocol sensors.

TL;DR: The method was successfully applied to the detection and determination of these pesticides in real water samples, without sample preparation steps, and the accuracy of the method was 94.9%.

TL;DR: Electroactive hydrogel composites that physically entrap enzymes within their matrices for biosensor construction and chemically stimulated controlled release demonstrate the potential of such systems to function as a chemically-synthesized artificial pancreas.

TL;DR: Preliminary experimental results showing the antibody activity against myoglobin, detected with a microcantilever based sensor prototype confirmed the motivations and potentialities of the proposed immunosensing technique.

TL;DR: In this article, a two-point calibration procedure was proposed for continuous glucose monitoring, where one point calibration was performed before each meal, and the other was performed after each meal.

TL;DR: The results demonstrate that further progress aimed at long term biostability of implanted biosensors needs to solve technological problems and the serial production of sensors with really comparable qualities as a prerequisite for clinical trials.

TL;DR: The aim of designing a highly specific and sensitive, re-usable affinity sensor for antigens on the basis of an integrated optical Mach-Zehnder interferometer is based on a proper understanding of the characteristics of the entire binding matrix architecture.

TL;DR: These biosensors exhibited an exquisite specificity evidenced by their ability to discriminate antigens, the structures of which differ only by the isomeric form of di-deoxyhexose.

TL;DR: Hemoglobin in the lipid film exhibited elegant catalytic activity for electrochemical reduction of H( 2)O(2), based which a unmediated biosensor for H(2)O (2) was developed.

TL;DR: An increased number of repeated analyses of 750 samples during 230 days for glucose and 400 samples during 40 days of operation for sucrose have been achieved, which will improve the operating cost effectiveness of the biosensor.

TL;DR: This work investigates a new method combining microphotolithographical techniques with laser excimer beam technology to create surfaces with well defined 3-D microdomains in order to delineate critical microscopic surface features governing material-cell interaction.

TL;DR: A prototype of a portable optical immunosensor (called river analyser) has been developed and can be applied for the monitoring of surface water quality, where at least three analytes can be detected simultaneously in one sample.

TL;DR: O(2) was electroreduced to water at 0.6 V (SHE) near neutral pH on the "wired" Pleurotus ostreatus laccase cathode and substitution of the C. hirsutus l Accase by laccases from P. ostreats allows the upward extension of the pH range of O(2).

TL;DR: Several effects previously determined from kinetic measurements of the enzyme reconstituted in liposomes such as saturation with respect to the substrate (ATP), inhibition by venturicidin, activation by a positive potential pulse and increase of the proton current as a function of increasingly negative potentials have been confirmed also for this tethered membrane system.

TL;DR: A glucose biosensor was constructed by electrochemical adsorption of glucose oxidase (GOD) in microporous polyacrylonitrile (PAN), which was synthesized by single rare-earth catalyst-Y(OAr)(3.)

TL;DR: In this article, a two-point calibration procedure consists of the determination of a sensor sensitivity S and of a background current I(o) by plotting two values of the sensor signal versus the concomitant blood glucose concentrations.

TL;DR: A novel approach for the label-free detection of molecular interactions is presented in which a colorimetric resonant grating is used as a surface binding platform, capable of detecting the addition and removal of small molecules as they interact with receptor molecules on the sensor surface or enzymes in the solution surrounding the sensor.

TL;DR: Urease immobilized on PNVK/SA LB films, characterized using FTIR and UV-visible spectroscopy, was found to exhibit increased stability over a wide pH (6.5-8.5) and temperature (25-50 degrees C) range.

TL;DR: Two principally different applications are considered, effects monitoring with biological components as targets for bioeffective substances, among them endocrine disruptors; and immunochemical analysis employing antibodies as binding proteins for a wide variety of analytes such as pesticides.