Journal ArticleDOI
Biological behavior of pre‐osteoblasts on natural hydroxyapatite: A study of signaling molecules from attachment to differentiation
Willian Fernando Zambuzzi,Willian Fernando Zambuzzi,Willian Fernando Zambuzzi,Carmen Veríssima Ferreira,José Mauro Granjeiro,José Mauro Granjeiro,Hiroshi Aoyama +6 more
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TLDR
This study demonstrates that osteoblast response toward bovine bone HA is initially mediated by activation of focal adhesion components, culminating on actin-rearrangement executed by cofilin activation via rac-1.Abstract:
Several biomaterials have been widely used in bone regeneration in both orthopedic and oral surgeries. However, it is poorly understood how these biomaterials alter osteoblast phenotype. It prompted us to examine the involvement of signaling proteins during preosteoblast adhesion (attachment), proliferation, and differentiation on natural hydroxyapatite (HA) from bovine bone. Our results indicated that natural HA is able to promote osteoblast adhesion, proliferation, and differentiation. The osteoblast/HA interaction requires phosphorylation of tyrosine residues of focal adhesion kinase, Src, and Paxillin upon integrin activation, which culminates in the control of cofilin phosphorylation (at serine 03) via rac-1 activation. In part, these signaling pathways are responsible for actin-rearrangement, responsible to adapt cell-shape on HA particles. In regarding to osteoblast differentiation, we showed that natural HA favored extracellular matrix remodeling by stimulating matrix metalloproteinase activities and alkaline phosphatase activity. Overall, this study demonstrates that osteoblast response toward bovine bone HA is initially mediated by activation of focal adhesion components, culminating on actin-rearrangement executed by cofilin activation via rac-1. Moreover, bovine bone HA provided an excellent microenvironment for osteoblast activity, since adhesion to differentiation.read more
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Silk fibroin/hydroxyapatite composites for bone tissue engineering.
Mehdi Farokhi,Fatemeh Mottaghitalab,Saeed Samani,Mohammad Ali Shokrgozar,Subhas C. Kundu,Rui L. Reis,Yousef Fatahi,David L. Kaplan +7 more
TL;DR: Bl blending SF and HAp combines the useful properties of both materials as bone constructs for tissue engineering, the subject of this review.
Journal ArticleDOI
Composite scaffolds of nano-hydroxyapatite and silk fibroin enhance mesenchymal stem cell-based bone regeneration via the interleukin 1 alpha autocrine/paracrine signaling loop
Hua Liu,Guo Wei Xu,Ya Fei Wang,Hong Shi Zhao,Si Xiong,Yan Wu,Boon Chin Heng,Chengrui An,Gang Hua Zhu,Ding Hua Xie +9 more
TL;DR: The biocompatibility and superior osteoinductivity of nHAp-SF scaffolds versus SF scaffolds both in vitro and on a calvarial defect model in vivo were confirmed and complemented the understanding of the underlying mechanisms of biomaterial osteoInductivity.
Journal ArticleDOI
Micro-arc oxidation as a tool to develop multifunctional calcium-rich surfaces for dental implant applications.
Ana R. Ribeiro,F. S. Oliveira,Leonardo C Boldrini,Paulo Emílio Corrêa Leite,Priscila Falagan-Lotsch,Adriana Linhares,Willian Fernando Zambuzzi,Benjamin Fragneaud,A.P.C. Campos,C.P. Gouvea,Braulio S. Archanjo,C. A. Achete,E. Marcantonio,Luís Augusto Rocha,José Mauro Granjeiro +14 more
TL;DR: High-resolution techniques allowed to understand that osteoblasts adhered less in the crystalline-rich regions while they preferentially adhere and spread over in the Ca-rich amorphous oxide layer, which might lead to faster osseointegration.
Nanomaterials and nanotechnology for skin tissue engineering.
Aezeden Mohamed,Malcolm Xing +1 more
TL;DR: A comprehensive review of the prospects for nanotechnology and nanomaterials to close this gap by mimicking surface properties for reconstruction of a variety of skin tissues.
Journal ArticleDOI
Nanometer scale titanium surface texturing are detected by signaling pathways involving transient FAK and Src activations
Willian Fernando Zambuzzi,Estevam A. Bonfante,Ryo Jimbo,Mariko Hayashi,Martin Andersson,Gutemberg Gomes Alves,Esther Rieko Takamori,Paulo José I. Beltrão,Paulo G. Coelho,José Mauro Granjeiro +9 more
TL;DR: It can be suggested that signaling pathways involving both FAK and Src could provide biomarkers to predict osteoblast adhesion onto different surfaces as a function of titanium surfaces physico/chemical configuration and protein adsorption.
References
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Cofilin phosphorylation by LIM-kinase 1 and its role in Rac-mediated actin reorganization
Neng Yang,Osamu Higuchi,Kazumasa Ohashi,Kyoko Nagata,Atsushi Wada,Kenji Kangawa,Eisuke Nishida,Kensaku Mizuno,Kensaku Mizuno +8 more
TL;DR: It is shown that LIM-kinase 1 (LIMK-1), a serine/threonine kinase containing LIM and PDZ domains, phosphorylates cofilin at Ser’3, both in vitro and in vivo, which indicates that LIMK- 1 participates in Rac-mediated actin cytoskeletal reorganization, probably by phosphoryLating co Filin.
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Functional atlas of the integrin adhesome
TL;DR: Examination of the adhesome network motifs reveals a relatively small number of key motifs, dominated by three-component complexes in which a scaffolding molecule recruits both a signalling molecule and its downstream target.