Journal ArticleDOI
Biomaterials with permanent hydrophilic surfaces and low protein adsorption properties.
B E Rabinow,Y S Ding,C Qin,M L McHalsky,J H Schneider,K A Ashline,T L Shelbourn,Ralph M. Albrecht +7 more
TLDR
Permanency of the hydrophilic surfaces so generated was confirmed by surface contact angle experiments and total organic carbon leachables analysis of the aqueous contacting solutions, as determined by the Fluoraldehyde procedure.Abstract:
Low protein adsorbing polymer films have been prepared with which to fabricate intravenous containers, designed for compatibility with low concentrations of protein drugs. The material is economically manufactured utilizing physical melt blending of water-soluble surface-modifying polymers (PEO, PEOX, PVA, and PNVP) with a base polymer (EVA, PP, PETG, PMMA, SB, and nylon). Permanency of the hydrophilic surfaces so generated was confirmed by surface contact angle experiments and total organic carbon leachables analysis of the aqueous contacting solutions. Binding of IgG, albumin and insulin was studied. A sixfold reduction of protein adsorption was obtained by adding 5% PVA13K to EVA, for IgG at a bulk concentration of 2.5 ppm. Surface bound protein measured by micro-BCA colorimetry, agreed with the solution protein lost, as determined by the Fluoraldehyde procedure. Imaging of the protein exposed plastic surfaces by silver enhanced protein conjugated gold staining agreed with the quantitative assay determinations.read more
Citations
More filters
Journal ArticleDOI
Zwitterionic SAMs that Resist Nonspecific Adsorption of Protein from Aqueous Buffer
TL;DR: This paper describes the use of surface plasmon resonance spectroscopy and self-assembled monolayers (SAMs) of alkanethiols on gold to evaluate the ability of surfaces terminating in different combinations of charged groups to resist the nonspecific adsorption of proteins from aqueous buffer.
Journal ArticleDOI
Protein adsorption on solid surfaces
Vladimir Hlady,Jos Buijs +1 more
TL;DR: The research field of protein adsorption on surfaces appears to be as popular as ever, with several hundred published papers tackled problems ranging from fundamental aspects of protein surface interactions to applied problems of surface blood compatibility and protein surface immobilization.
Journal ArticleDOI
A collagenous protective coat enables Metarhizium anisopliae to evade insect immune responses.
TL;DR: Studies with staining reagents and hemocyte monolayers showed that MCL1 functions as an antiadhesive protective coat because it masks antigenic structural components of the cell wall such as beta-glucans, and because its hydrophilic negatively charged nature makes it unattractive to hemocytes.
Journal ArticleDOI
Bioapplications of hyperbranched polymers
TL;DR: The present review highlights the contribution of HBPs to biological and biomedical fields with intent to aid the researchers in exploring HBPs for bioapplications.
Journal ArticleDOI
Platelet adhesion onto segmented polyurethane film surfaces modified by addition and crosslinking of PEO-containing block copolymers
TL;DR: It was observed that addition of a small amount (5 wt% based on PU) of the PEO additives resulted in a considerable change of surface characteristics, without significant changes of bulk properties of the films.
References
More filters
Journal ArticleDOI
Controlled nucleation for the regulation of the particle size in monodisperse gold suspensions
TL;DR: In this article, a series of monodisperse suspensions of the same chemical composition but of rather different particle sizes was used to study particle size dependent phenomena, such as Brownian motion, light scattering, sedimentation and electrophoresis of small particles.
Book
Colloidal Gold: Principles, Methods, and Applications
TL;DR: Some basic concepts for the choice methods for the production of monoclonal and polyclonal antibodies and the immunocytochemistry of antigen-antibody reactions at interfaces are presented.
Journal ArticleDOI
Protein-resistant surfaces prepared by PEO-containing block copolymer surfactants.
TL;DR: The data presented indicate that adsorption of the surfactants on LDPE is dependent on the molecular geometry of theSurfactants suitable for the preparation of PEO-rich surfaces and possible mechanisms for their protein resistance are discussed.
Journal ArticleDOI
Solution technique to incorporate polyethylene oxide and other water-soluble polymers into surfaces of polymeric biomaterials.
Neil P. Desai,Jeffrey A. Hubbell +1 more
TL;DR: It was concluded that PEO 18,500 had a chain length that was optimal, using this technique for surface incorporation, to reduce protein adsorption and hence prevent protein-mediated biological interactions.
Journal ArticleDOI
Biological responses to polyethylene oxide modified polyethylene terephthalate surfaces
Neil P. Desai,Jeffrey A. Hubbell +1 more
TL;DR: It was concluded that PEO of molecular weights neighboring 18,500 and higher was effective in reducing protein adsorption and cellular interactions on these surfaces.