Bovine sperm forward motility protein. Partial purification and characterization.
Ted S. Acott,Dale D. Hoskins +1 more
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TLDR
It is suggested that this protein is involved in the development of the capacity for motility as sperm traverse the epididymis as well as other studies on the activity, properties, and distribution of forward motility protein in bovine body fluids.About:
This article is published in Journal of Biological Chemistry.The article was published on 1978-10-10 and is currently open access. It has received 123 citations till now. The article focuses on the topics: Motility & Affinity chromatography.read more
Citations
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Journal ArticleDOI
Major Proteins of Bovine Seminal Plasma Bind to the Low-Density Lipoprotein Fraction of Hen's Egg Yolk
TL;DR: It is proposed that the sequestration of BSP proteins of SP by LDF may represent the major mechanism of sperm protection by EY, and it is shown that the B SP proteins bind to the low-density fraction (LDF), a lipoprotein component of the EY extender.
Journal ArticleDOI
Characterization and androgen-dependence of proteins associated with luminal fluid and spermatozoa in the rat epididymis
D. E. Brooks,S. J. Higgins +1 more
TL;DR: Several changes were observed in the relative amounts of specific proteins extracted from spermatozoa from different regions of the epididymis and several of these proteins had molecular weights identical with those in luminal fluid and there was no evidence for any substantial binding to spermatozosa of the pre-albumin proteins (B-E) of luminal fluids.
Journal ArticleDOI
Sperm surface galactosyltransferase activities during in vitro capacitation
Barry D. Shur,N G Hall +1 more
TL;DR: The results define a molecular mechanism for one aspect of sperm capacitation, and help explain why removal of “decapacitation factos” is a necessary prerequisite for sperm binding to the zona pellucida.
Book ChapterDOI
Secretion and endocytosis in the male reproductive tract: a role in sperm maturation
TL;DR: An important concept discussed in this chapter is that the spermatozoon itself may contribute to its own maturation providing that the appropriate conditions of its external milieu are met by the secretory and endocytic activities along the excurrent reproductive duct system.
Journal ArticleDOI
Lectins as probes of the spermatozoon surface.
TL;DR: Changes in the patterns of lectin binding have been noted during sperm maturation in the epididymis, during capacitation, and after the acrosome reaction, and these studies contribute to the understanding of mechanism that may mediate these transformations.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal ArticleDOI
Maturation of the head of bacteriophage T4. I. DNA packaging events.
Ulrich K. Laemmli,M Favre +1 more
TL;DR: Pulse-chase experiments in wild-type and mutant phage-infected cells provide evidence that the following particles called prohead I, II and III are successive precursors to the mature heads as discussed by the authors.
Journal ArticleDOI
The Gross Conformation of Protein-Sodium Dodecyl Sulfate Complexes
TL;DR: The data suggest a possible model for the conformation of membrane proteins and their interactions with phospholipid, and the empirical observation that proteins dissolved in aqueous solutions containing high concentrations of sodium dodecyl sulfate have electrophoretic mobilities on polyacrylamide gels which are a unique function of their molecular weights.
Journal ArticleDOI
The Estimation of Polypeptide Chain Molecular Weights by Gel Filtration in 6 M Guanidine Hydrochloride
TL;DR: Gel filtration of reduced protein polypeptide chains in 6 m guanidine hydrochloride has been evaluated as a means of estimating protein or protein subunit molecular weights as suggested by Davison and excellent precision and accuracy can be obtained.
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