Calibration-free quantification of absolute oxygen saturation based on the dynamics of photoacoustic signals
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TLDR
This Letter circumvents the requirement for calibration-free quantification of absolute oxygen saturation by taking advantage of the dynamics in sO₂ by using the ratio of photoacoustic amplitudes measured at different sO ₂ states to cancels the contribution from optical fluence.Abstract:
Photoacoustic tomography (PAT) is a hybrid imaging technique that has broad preclinical and clinical applications. Based on the photoacoustic effect, PAT directly measures specific optical absorption, which is the product of the tissue-intrinsic optical absorption coefficient and the local optical fluence. Therefore, quantitative PAT, such as absolute oxygen saturation (sO_2) quantification, requires knowledge of the local optical fluence, which can only be estimated through invasive measurements or sophisticated modeling of light transportation. In this Letter, we circumvent this requirement by taking advantage of the dynamics in sO_2. The new method works when the sO_2 transition can be simultaneously monitored with multiple wavelengths. For each wavelength, the ratio of photoacoustic amplitudes measured at different sO_2 states is utilized. Using the ratio cancels the contribution from optical fluence and allows calibration-free quantification of absolute sO_2. The new method was validated through both phantom and in vivo experiments.read more
Citations
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Photoacoustic tomography: principles and advances.
TL;DR: The photoacoustic tomography (PAT) is an emerging imaging modality that shows great potential for preclinical research and clinical practice as mentioned in this paper, which is based on the acoustic detection of optical absorption from either endogenous chromophores, such as oxy-hemoglobin and deoxy-hemoglobaloglobin, or exogenous contrast agents such as organic dyes and nanoparticles.
Journal ArticleDOI
Single-impulse Panoramic Photoacoustic Computed Tomography of Small-animal Whole-body Dynamics at High Spatiotemporal Resolution.
Lei Li,Liren Zhu,Cheng Ma,Li Lin,Li Lin,Junjie Yao,Lidai Wang,Konstantin Maslov,Ruiying Zhang,Wanyi Chen,Junhui Shi,Lihong V. Wang +11 more
TL;DR: It is demonstrated that standalone single-impulse photoacoustic computed tomography (SIP-PACT) mitigates these limitations by combining high spatiotemporal resolution, deep penetration, anatomical, dynamical and functional contrasts, and full-view fidelity.
Journal ArticleDOI
Tutorial on photoacoustic tomography
TL;DR: This work focuses on PAT’s basic principles, major implementations, imaging contrasts, and recent applications, and examines its use for multiscale anatomical, functional, and molecular imaging of biological tissues.
Journal ArticleDOI
Single-breath-hold photoacoustic computed tomography of the breast.
Li Lin,Li Lin,Peng Hu,Junhui Shi,Catherine M. Appleton,Konstantin Maslov,Lei Li,Ruiying Zhang,Lihong V. Wang +8 more
TL;DR: SBH-PACT clearly reveals tumors by observing higher blood vessel densities associated with tumors at high spatial resolution, showing early promise for high sensitivity in radiographically dense breasts.
Journal ArticleDOI
Small-Animal Whole-Body Photoacoustic Tomography: A Review
Jun Xia,Lihong V. Wang +1 more
TL;DR: The rapidly growing field of small-animal whole-body Photoacoustic tomography is reviewed and studies done in the past decade are highlighted.
References
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Lihong V. Wang,Song Hu +1 more
TL;DR: A review of the state of the art of photoacoustic tomography for both biological and clinical studies can be found in this paper, where the authors discuss the current state-of-the-art and discuss future prospects.
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Journal ArticleDOI
MCML—Monte Carlo modeling of light transport in multi-layered tissues
TL;DR: A Monte Carlo model of steady-state light transport in multi-layered tissues (MCML) has been coded in ANSI Standard C; therefore, the program can be used on various computers as mentioned in this paper.
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TL;DR: Because malignant tumors no longer execute functions necessary for homeostasis (such as the production of adequate amounts of adenosine triphosphate), the physiology-based definitions of the term "hypoxia" are not necessarily valid for malignant tumor patients.
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Minghua Xu,Lihong V. Wang +1 more
TL;DR: In this article, a universal back-projection algorithm for three-dimensional photoacoustic computed tomography is presented for three types of imaging geometries: planar, spherical, and cylindrical surfaces.