Open AccessJournal Article
Carbohydrate Changes in Glycoproteins of a Poorly Metastasizing Wheat Germ Agglutinin-resistant Melanoma Clone
TLDR
Binding of 125I-labeled lectins to total cellular proteins on polyacrylamide gels following electrophoresis showed that the major wheat germ agglutinin-binding components of F1 cells were altered in Wa-4 cells, suggesting a possible basis for the glycosylation change.Abstract:
Glycoproteins of a metastasizing line of B16 mouse melanoma and a poorly metastasizing wheat germ agglutinin-resistant clone were compared. Cell surface proteins and glycoproteins were isotopically labeled by lactoperoxidase-catalyzed iodination and by NaB 3 H 4 reduction after oxidation by periodate or galactose oxidase and subsequently analyzed by gel electrophoresis and autoradiography. Differences were observed in the relative mobilities of several major cell surface components. Binding of 125 I-labeled lectins to total cellular proteins on polyacrylamide gels following electrophoresis showed that the major wheat germ agglutinin-binding components of F 1 cells were altered in Wa-4 cells. Similar differences were not observed in concanavalin A-binding components. Total cellular glycopeptides were analyzed after separation into structurally distinct classes. The acidic “complex” N -glycosidic glycopeptides from the resistant cells were of lower molecular weight than those from the parent cells. No differences were observed among the mannose-rich N -glycosidic glycopeptides or the alkali-labile O -glycosidic oligosaccharides. Structural studies involving methylation analysis revealed that in the altered glycopeptides of the resistant cells the amount of neuraminic acid residues was decreased to one-half, concomitant with an increase in the amount of fucose. The lost sialic acid was bound to C-3 of galactose, whereas the increased fucose was found on C-3 of 4-substituted N -acetylglucosamine. A possible basis for the glycosylation change and its relation to the biological behavior are discussed.read more
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References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
A Film Detection Method for Tritium‐Labelled Proteins and Nucleic Acids in Polyacrylamide Gels
TL;DR: A simple method for detecting 3H in polyacrylamide gels by scintillation autography (fluorography) using X-ray film, which is ten times more sensitive than conventional autoradiography of isotopes with higher emission energies.
Journal ArticleDOI
Selection of successive tumour lines for metastasis.
TL;DR: The pathogenesis of metastasis begins with the invasion of tissues, blood vessels and/or lymphatics by cells originating from a primary cancer, and most tumour emboli are initially arrested in the first capillary bed encountered, but some recirculate and are trapped in other organs.
Journal ArticleDOI
Enzymatic iodination of polypeptides with 125I to high specific activity.
J.I. Thorell,B.G. Johansson +1 more
TL;DR: Polypeptide hormones radioiodinated by this technique retained their immunological reactivity and were used in radioimmunoassays with good results.
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