Journal ArticleDOI
Changes in growth, lipid peroxidation and some key antioxidant enzymes in chickpea genotypes under salt stress
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TLDR
The results indicate that NaCl-induced oxidative stress hampers the normal functioning of the cell and indicates that the genes involved in the antioxidative process are triggered by oxidative stress induced by environmental change.Abstract:
The present study was conducted to evaluate the effect of NaCl on growth and some key antioxidants in chickpea. Eight genotypes of chickpea were grown hydroponically for 15 days and then treated with different concentrations of salt [0 mM (T0), 25 mM (T1), 50 mM (T2), 75 mM (T3), and 100 mM (T4)]. Salinity showed marked changes in growth parameters (fresh and dry weight of root and shoot). The level of lipid peroxidation was measured by estimating malondialdehyde content. Lipid peroxidation increases with the increase in NaCl concentration in all genotypes but salt-tolerant genotypes (SKUA-06 and SKUA-07) were least affected as compared to other genotypes. The chlorophyll content was also affected with elevated levels of NaCl. Increased concentration of salt increased the activity of antioxidant enzymes like superoxide dismutase (SOD), catalase (CAT), ascorbate peroxidase (APX) and glutathione reductase in all chickpea genotypes but maximum activity was observed in salt-tolerant (SKUA-06 and SKUA-07) genotypes. Two genotypes of salt-tolerant and salt-sensitive varieties were analyzed further by real time PCR which revealed that the expression of SOD, APX and CAT genes were increased by NaCl in the salt-tolerant variety. The enhancement in tolerance against salt stress indicates that the genes involved in the antioxidative process are triggered by oxidative stress induced by environmental change. The results indicate that NaCl-induced oxidative stress hampers the normal functioning of the cell. The efficient antioxidants play a great role in mitigating the effect of NaCl stress in chickpea. This screening of NaCl-tolerant genotypes of chickpea can be performed on salt-affected land.read more
Citations
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Journal ArticleDOI
Portraying mechanics of plant growth promoting rhizobacteria (PGPR): A review
TL;DR: This review is focused on cogent mechanics employed by PGPR that assists plant to sustain healthy growth and the PGPR-based products which have been commercially developed exploiting these mechanics.
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Nitric Oxide Mitigates Salt Stress by Regulating Levels of Osmolytes and Antioxidant Enzymes in Chickpea
Parvaiz Ahmad,Arafat Abdel Hamed Abdel Latef,Arafat Abdel Hamed Abdel Latef,Abeer Hashem,Elsayed Fathi Abd_Allah,Salih Gucel,Lam-Son Phan Tran +6 more
TL;DR: NO has capability to mitigate the adverse effects of high salinity on chickpea plants by improving LRWC, photosynthetic pigment biosyntheses, osmolyte accumulation and antioxidative defense system.
Journal ArticleDOI
Exogenous jasmonic acid can enhance tolerance of wheat seedlings to salt stress.
TL;DR: It is suggested that JA could effectively protect wheat seedlings from salt stress damage by enhancing activities of antioxidant enzymes and the concentration of antioxidative compounds to quench the excessive reactive oxygen species caused by salt stress and presented a practical implication for wheat cultivation in salt-affected soils.
Journal ArticleDOI
Role of Trichoderma harzianum in mitigating NaCl stress in Indian mustard (Brassica juncea L) through antioxidative defense system.
Parvaiz Ahmad,Abeer Hashem,Elsayed Fathi Abd-Allah,Abdulaziz A. Alqarawi,Riffat John,Dilfuza Egamberdieva,Salih Gucel +6 more
TL;DR: TH proved to be very beneficial in imparting resistance to the mustard plants against NaCl stress through improved uptake of essential elements, modulation of osmolytes and antioxidants and reduction in the accumulation by addition of TH to mustard seedlings.
Journal ArticleDOI
Calcium and Potassium Supplementation Enhanced Growth, Osmolyte Secondary Metabolite Production, and Enzymatic Antioxidant Machinery in Cadmium-Exposed Chickpea (Cicer arietinum L.).
Parvaiz Ahmad,Parvaiz Ahmad,Arafat Abdel Hamed Abdel Latef,Arafat Abdel Hamed Abdel Latef,Elsayed Fathi Abd_Allah,Abeer Hashem,Maryam Sarwat,Naser A. Anjum,Salih Gucel +8 more
TL;DR: This investigation suggests that application of Ca and/or K can efficiently minimize Cd-toxicity and eventually improve health and yield in C. arietinum by the cumulative outcome of the enhanced contents of organic solute, secondary metabolites, mineral elements, and activity of antioxidant defense enzymes.
References
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Book ChapterDOI
Catalase in vitro
TL;DR: In this article, the catalytic activity of catalase has been investigated using ultraviolet (UV) spectrophotometry and Titrimetric methods, which is suitable for comparative studies for large series of measurements.
Journal ArticleDOI
Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
TL;DR: The staining procedure for localizing superoxide dismutase on polyacrylamide electrophoretograms has been applied to extracts obtained from a variety of sources and could thus be assayed either in crude extracts or in purified protein fractions.
Journal ArticleDOI
REACTIVE OXYGEN SPECIES: Metabolism, Oxidative Stress, and Signal Transduction
Klaus Apel,Heribert Hirt +1 more
TL;DR: The mechanisms of ROS generation and removal in plants during development and under biotic and abiotic stress conditions are described and the possible functions and mechanisms for ROS sensing and signaling in plants are compared with those in animals and yeast.
Journal ArticleDOI
Oxidative stress, antioxidants and stress tolerance
TL;DR: Key steps of the signal transduction pathway that senses ROIs in plants have been identified and raise several intriguing questions about the relationships between ROI signaling, ROI stress and the production and scavenging ofROIs in the different cellular compartments.
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