Changes in Leaf Anatomical Traits Enhanced Photosynthetic Activity of Soybean Grown in Hydroponics with Plant Growth-Promoting Microorganisms.
Roberta Paradiso,Carmen Arena,Veronica De Micco,Maria Giordano,Giovanna Aronne,Stefania De Pascale +5 more
TLDR
It is confirmed that PGPMs may confer benefits in photosynthetic traits of soybean plants even in hydroponics (i.e., NFT), with positive effects on growth and seed production, prefiguring potential application of beneficial microorganisms in plant cultivation in hydoponics.Abstract:
The use of hydroponic systems for cultivation in controlled climatic conditions and the selection of suitable genotypes for the specific environment help improving crop growth and yield. We hypothesized that plant performance in hydroponics could be further maximised by exploiting the action of plant growth-promoting organisms (PGPMs). However, the effects of PGPMs on plant physiology have been scarcely investigated in hydroponics. Within a series of experiments aimed to identify the best protocol for hydroponic cultivation of soybean [Glycine max (L.) Merr.], we evaluated the effects of a PGPMs mix, containing bacteria, yeasts, mycorrhiza and trichoderma beneficial species on leaf anatomy, photosynthetic activity and plant growth of soybean cv. ‘Pr91m10’in closed nutrient film technique (NFT). Plants were grown in a growth chamber under semi-aseptic conditions and inoculated at seed, seedling and plant stages, and compared to non-inoculated (control) plants. Light and epi-fluorescence microscopy analyses showed that leaves of inoculated plants had higher density of smaller stomata (297 vs 247 n/mm2), thicker palisade parenchyma (95.0 vs 85.8 μm), and larger intercellular spaces in the mesophyll (57.5% vs 52.2%), compared to non-inoculated plants. The modifications in leaf functional anatomical traits affected gas exchanges; in fact starting from the reproductive phase, the rate of leaf net photosynthesis was higher in inoculated compared to control plants (8.69 vs 6.13 μmol CO2 m-2 s-1 at the beginning of flowering). These data are consistent with the better maximal PSII photochemical efficiency observed in inoculated plants(0.807 vs 0.784 in control); conversely no difference in leaf chlorophyll content was found. The PGPM-induced changes in leaf structure and photosynthesis lead to an improvement of plant growth (+29.9% in plant leaf area) and seed yield (+36.9%) compared to control. Our results confirm that PGPMs may confer benefits in photosynthetic traits of soybean plants even in hydroponics (i.e. NFT), with positive effects on growth and seed production, prefiguring potential application of beneficial microorganisms in plant cultivation in hydroponics.read more
Citations
More filters
Journal ArticleDOI
Muscle Oximetry in Sports Science: A Systematic Review
Stéphane Perrey,Marco Ferrari +1 more
TL;DR: Overall, NIRS information can be used as a marker of skeletal muscle oxidative capacity and for analyzing muscle performance factors and there is still the need for further instrumental development and randomized/longitudinal trials to support the detailed advantages of muscle oximetry utilization in sports science.
Journal ArticleDOI
Endophytic strains of Trichoderma increase plants’ photosynthetic capability
TL;DR: Reductions in global greenhouse gas levels can be accelerated by giving incentives for climate‐friendly carbon farming and carbon cap‐and‐trade programmes that reward practices transferring carbon from the atmosphere into the soil, also enhancing soil fertility and agricultural production.
Journal ArticleDOI
PGPR-mediated induction of systemic resistance and physiochemical alterations in plants against the pathogens: Current perspectives.
Mukesh Meena,Mukesh Meena,Prashant Swapnil,Prashant Swapnil,Kumari Divyanshu,Sunil Kumar,Harish,Yashoda Nandan Tripathi,Andleeb Zehra,Avinash Marwal,Ram Sanmukh Upadhyay +10 more
TL;DR: The PGPR‐mediated innovative methods are discussed, focusing on the mode of action of compounds authorized that may be significant in the development contributing to enhance plant growth, disease resistance, and serve as an efficient bioinoculants for sustainable agriculture.
Journal ArticleDOI
Plant growth-promoting endophyte Piriformospora indica alleviates salinity stress in Medicago truncatula.
TL;DR: The results demonstrate that tolerance to salinity stress was conferred by P. indica in M. truncatula via accumulation of osmoprotectant, stimulating antioxidant enzymes and the expression of defense-related genes.
Journal ArticleDOI
Halotolerant rhizobacteria Pseudomonas pseudoalcaligenes and Bacillus subtilis mediate systemic tolerance in hydroponically grown soybean (Glycine max L.) against salinity stress
Humaira Yasmin,Sana Naeem,Murk Bakhtawar,Zahra Jabeen,Asia Nosheen,Rabia Naz,Rumana Keyani,Saqib Mumtaz,Muhammad Nadeem Hassan +8 more
TL;DR: P. pseudoalcaligenes inoculated soybean plants showed tolerance by the increased activities of defense related system such as ion transport, antioxidant enzymes, proline and MDA content in shoots and roots by inducing tolerance in soybean against salinity stress in hydroponics by enhancing plant biomass, relative water content and osmolytes.
References
More filters
Journal ArticleDOI
The relationship between the quantum yield of photosynthetic electron transport and quenching of chlorophyll fluorescence
TL;DR: In this article, the quantum yield of non-cyclic electron transport was found to be directly proportional to the product of the photochemical fluorescence quenching (qQ) and the efficiency of excitation capture by open Photosystem II (PS II) reaction centres (Fv/Fm).
Journal ArticleDOI
Chlorophyll fluorescence—a practical guide
Kate Maxwell,Giles N. Johnson +1 more
TL;DR: An introduction for the novice into the methodology and applications of chlorophyll fluorescence is provided and a selection of examples are used to illustrate the types of information that fluorescence can provide.
Journal ArticleDOI
New spectrophotometric equations for determining chlorophylls a, b, c1 and c2 in higher plants, algae and natural phytoplankton
S.W. Jeffrey,G.F. Humphrey +1 more
TL;DR: New equations are presented for spectrophotometric determination of chlorophylls, based on revised extinction coefficients of chloropylls a, b, c1 and c2, which may be used for determining chlorophyLLs a and b in higher plants and green algae.
Journal ArticleDOI
Plant growth promoting rhizobacteria as biofertilizers
TL;DR: This review focuses on the known, the putative, and the speculative modes-of-action of PGPR, which include fixing N2, increasing the availability of nutrients in the rhizosphere, positively influencing root growth and morphology, and promoting other beneficial plant–microbe symbioses.