Journal ArticleDOI
Characterisation of ultraviolet-B-induced inflammation as a model of hyperalgesia in the rat.
Thomas Bishop,David W. Hewson,Ping K. Yip,M S Fahey,David Dawbarn,Antony R. Young,Stephen B. McMahon +6 more
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TLDR
It is concluded that UVB inflammation produces a dose‐dependent hyperalgesic state sensitive to established analgesics, which suggests thatUVB inflammation in the rat may represent a useful translational tool in the study of pain and the testing of analgesic agents.Abstract:
In humans, the acute inflammatory reaction caused by ultraviolet (UV) radiation is well studied and the sensory changes that are found have been used as a model of cutaneous hyperalgesia. Similar paradigms are now emerging as rodent models of inflammatory pain. Using a narrowband UVB source, we irradiated the plantar surface of rat hind paws. This produced the classical feature of inflammation, erythema, and a significant dose-dependent reduction in both thermal and mechanical paw withdrawal thresholds. These sensory changes peaked 48 h after irradiation. At this time there is a graded facilitation of noxious heat evoked (but not basal) c-fos-like immunoreactivity in the L4/5 segments of the spinal cord. We also studied the effects of established analgesic compounds on the UVB-induced hyperalgesia. Systemic as well as topical application of ibuprofen significantly reduced both thermal and mechanical hyperalgesia. Systemic morphine produced a dose-dependent and naloxone sensitive reversal of sensory changes. Similarly, the peripherally restricted opioid loperamide also had a dose-dependent anti-hyperalgesic effect, again reversed by naloxone methiodide. Sequestration of NGF, starting at the time of UVB irradiation, significantly reduced sensory changes. We conclude that UVB inflammation produces a dose-dependent hyperalgesic state sensitive to established analgesics. This suggests that UVB inflammation in the rat may represent a useful translational tool in the study of pain and the testing of analgesic agents.read more
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Journal ArticleDOI
Genome-wide transcriptional profiling of skin and dorsal root ganglia after ultraviolet-B-induced inflammation.
John M. Dawes,Ana Antunes-Martins,James R. Perkins,Kathryn J. Paterson,Marco Sisignano,Ramona Schmid,Werner Rust,Tobias Hildebrandt,Gerd Geisslinger,Christine A. Orengo,David L.H. Bennett,Stephen B. McMahon +11 more
TL;DR: A strong positive and significant correlation in gene expression between the human and rat species re-emphasises the value of the UVB model as translational tool to study inflammatory pain.
Journal ArticleDOI
Anti-inflammatory and antioxidant effects of Aloe saponaria Haw in a model of UVB-induced paw sunburn in rats.
Mariane Arnoldi Silva,Gabriela Trevisan,Carin Hoffmeister,Mateus Fortes Rossato,Aline Augusti Boligon,Cristiani Isabel Banderó Walker,Jonatas Zeni Klafke,Sara Marchesan Oliveira,Cássia Regina Silva,Margareth Linde Athayde,Juliano Ferreira,Juliano Ferreira +11 more
TL;DR: Results demonstrate that topical A. saponaria treatment displayed anti-nociceptive and anti-inflammatory effects in a UVB-induced sunburn model, and these effects seem to be related to its antioxidant components.
Journal ArticleDOI
Corneal sensory nerve activity in an experimental model of UV keratitis.
TL;DR: S Sensitization of nociceptor and depression of cold thermoreceptor activity following UV radiation appear to result from an action of inflammatory mediators on TRP channels selectively expressed by sensory nerve terminals.
Journal ArticleDOI
Pain and inflammation in hidradenitis suppurativa correspond to morphological changes identified by high-frequency ultrasound.
TL;DR: Hidradenitis suppurativa is an inflammatory skin disease with a chronic intermittent course and the current classification systems used to categorize disease severity provide limited insight into the degree of inflammation and pain.
Journal ArticleDOI
Effects of intrathecal ketorolac on human experimental pain.
TL;DR: Intrathecal ketorolac reduced hypersensitivity when it was induced by a combination of ultraviolet burn plus intermittent heat and, according to one of the two analytical strategies, when it is induced by ultraviolet burn alone.
References
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Journal ArticleDOI
Ethical guidelines for investigations of experimental pain in conscious animals.
TL;DR: The Committee for Research and Ethical Issues of the International Association for the Study of Pain (IASP®) is concerned with the ethical aspects of studies producing experimental pain and any suffering it may cause in animals.
Journal ArticleDOI
A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia.
TL;DR: Both the thermal method and the Randall‐Selitto mechanical method detected dose‐related hyperalgesia and its blockade by either morphine or indomethacin, but the Thermal method showed greater bioassay sensitivity and allowed for the measurement of other behavioral parameters in addition to the nociceptive threshold.
Journal Article
A new and sensitive method for measuring thermal nociception in cutaneous hyperalgesia.
Journal ArticleDOI
Textbook of pain
Patrick D. Wall,Ronald Melzack +1 more
TL;DR: Part 1 Basic aspects: peripheral - peripheral neural mechnaisms of nociception, the course and termination of primary afferent fibres, teh pathophysiology of damaged peripheral nerves, functional chemistry ofPrimary afferent neurons central - the dorsal horn.