Journal ArticleDOI
Characteristics of fertile somatic hybrids of G. hirsutum L. and G. trilobum generated via protoplast fusion.
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TLDR
The results suggest that novel traits may be incorporated in cotton breeding programs through the production of somatic hybrids and the backcrossing of these plants with elite cultivars.Abstract:
Fertile somatic hybrids between tetraploid upland cotton G. hirsutum L. cv. Coker 312 and wild cotton G. trilobum were generated by symmetric electrofusion. Comparisons of morphology, combined with flow cytometric, RAPD, SRAP and AFLP analyses confirmed the hybrid nature of the regenerated plants. The hybrids differed morphologically from the parent plants. Flow cytometric analysis showed that the hybrids had DNA similar in amount to the total combined DNA content of the two parents, and the use of molecular markers revealed that the hybrids contained genomic fragments from both fusion parents, further indicating the hybrid nature of the regenerated plants. The stability of the morphological features of the hybrids was examined in following generations. The hexaploid fusion plants showed strong photosynthesis and a high expression level of some photosystem-related genes. Our results suggest that novel traits may be incorporated in cotton breeding programs through the production of somatic hybrids and the backcrossing of these plants with elite cultivars.read more
Citations
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Journal ArticleDOI
Progress in plant protoplast research
Tom Eeckhaut,Prabhu Shankar Lakshmanan,Dieter Deryckere,Erik Van Bockstaele,Johan Van Huylenbroeck +4 more
TL;DR: The study of reactive oxygen species effects and DNA (de)condensation, along with thorough phytohormone monitoring, are in this review the most promising research topics in the further strive for rationalization of protoplast regeneration.
Book ChapterDOI
SRAP Molecular Marker Technology in Plant Science
TL;DR: This work has shown that Restriction fragment length polymorphism (RFLP) is the first hybridization-based molecular marker system that was intensively used at the begin‐ ning of the molecular biology era in life science while hybridisation-based marker methods such as microarrays and diversity array technology (DArT) are used currently to detect sin‐ gle nucleotide polymorphisms (SNP).
Book ChapterDOI
Cotton (Gossypium hirsutum L.) Breeding Strategies
Saeed Rauf,Muhammad Asif Shehzad,Jameel M. Al-Khayri,Hafiz Muhammad Imran,Ijaz Rasool Noorka +4 more
TL;DR: Genome editing technique such as CRISPR/Cas is one of the emerging technologies to knock out genes or SNP (single nucleotide polymorphism) substitution at specific site with future prospects for the development of disease resistant crop cultivars.
Journal ArticleDOI
Citrus somatic hybrid: an alternative system to study rapid structural and epigenetic reorganization in allotetraploid genomes
Shi-Xiao Xu,Shi-Xiao Xu,Dong-Fang Cai,Feng-Quan Tan,Yan-Ni Fang,Kai-Dong Xie,Jude W. Grosser,Wen-Wu Guo +7 more
TL;DR: The results suggest that somatic hybrids are very useful in elucidating the immediate changes that occur in newly synthesized allotetraploid, and take on a greater divergence from the callus parent and a closer relationship to leaf parent in all groups of plants by dendrogram analysis based on AFLP or MSAP data.
Journal ArticleDOI
Chromosome elimination and introgression following somatic hybridization between bread wheat and other grass species
TL;DR: The interaction effect between donor species and irradiation time significantly affected the chromatin behaviors during somatic hybridization, and the polyploid A. elongatum behaved differently from diploid H. villosa, even though these two species are phylogenetically equidistant from wheat.
References
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Visualization of differential gene expression using a novel method of RNA fingerprinting based on AFLP : Analysis of gene expression during potato tuber development
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Journal ArticleDOI
A rapid method for extraction of cotton (Gossypium spp.) genomic DNA suitable for RFLP or PCR analysis
TL;DR: This work describes a procedure that consistently permits isolation of cotton genomic DNA of satisfactory size and quality for RFLP and PCR analysis, as well as for most routine cloning applications.