Journal ArticleDOI
Characterization and sorting of cells based on stiffness contrast in a microfluidic channel
TLDR
The proposed microfluidic device has potential to be used as a lab on chip diagnostic tool for sorting of diseased cells from healthy cells based on stiffness contrast, which was found to depend on the stiffness contrast.Abstract:
This paper reports the characterization and sorting of cells based on stiffness contrast. Cell stiffness is characterized in terms of elastic modulus, deformability index and hydrodynamic resistance. For different cell types, elastic modulus is measured using nanoindentation experiments on AFM and deformability index of cells is measured by hydrodynamic stretching of the cells in a flow focusing microchannel device. Hydrodynamic resistance of cells is obtained by measuring the excess pressure drop across a segment of a microchannel and correlated with cell size ρc and elastic modulus using a large set of experimental data. The highly-invasive malignant breast cancer cells MDA MB 231, non-invasive malignant breast cancer cells MCF 7, human promyelocytic leukaemia cells HL60 and the cervical cancer cells HeLa are considered in the present study. A microfluidic device with focusing and spacing control for stiffness based sorting of cells was designed and fabricated. Experiments were performed to demonstrate cell sorting and characterize the device performance in terms of sorting efficiency, which was found to depend on the stiffness contrast. The proposed device has potential to be used as a lab on chip diagnostic tool for sorting of diseased cells from healthy cells based on stiffness contrast.read more
Citations
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Journal ArticleDOI
A combined experimental and theoretical approach towards mechanophenotyping of biological cells using a constricted microchannel
TL;DR: A combined experimental and theoretical technique that enables the characterization of various mechanical properties of biological cells and leads to a new paradigm for mechanophenotyping of biological Cells.
Journal ArticleDOI
Quantitative phase microscopy of red blood cells during planar trapping and propulsion
Azeem Ahmad,Vishesh Dubey,Vijay Raj Singh,Jean-Claude Tinguely,Cristina Ionica Øie,Deanna Wolfson,Dalip Singh Mehta,Peter T. C. So,Balpreet Singh Ahluwalia +8 more
TL;DR: This work has combined quantitative phase microscopy and waveguide trapping techniques to study changes in RBC morphology during planar trapping and transportation.
Journal ArticleDOI
High-throughput assessment of mechanical properties of stem cell derived red blood cells, toward cellular downstream processing.
Ewa Guzniczak,Maryam Mohammad Zadeh,Fiona Dempsey,Melanie Jimenez,Henry Bock,Graeme Whyte,Nicholas Willoughby,Helen Bridle +7 more
TL;DR: Deformability and size changes in CD34+ cells, and expelled nuclei, during their differentiation process into red blood cells are characterized for the first time using Real-Time Deformability Cytometry and Atomic Force Microscopy.
Journal ArticleDOI
An investigation of the viscoelastic properties and the actin cytoskeletal structure of triple negative breast cancer cells.
TL;DR: The results suggest that the shear assay measurements of cell viscoelastic properties may be used as effective biomarkers for TNBC diagnosis and screening.
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Dual-neodymium magnet-based microfluidic separation device.
Hyeon Gi Kye,Byeong Seon Park,Jong Min Lee,Min Gyu Song,Han Gyeol Song,Christian D. Ahrberg,Bong Geun Chung +6 more
TL;DR: A microfluidic device with dual-neodymium magnet-based negative magnetophoresis for the separation of the microparticles and cells is developed and the separations of glioblastoma cancer cells and neural stem cells are demonstrated.
References
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