Characterization of anastomosis groups of binucleate Rhizoctonia species using restriction analysis of an amplified ribosomal RNA gene.

TLDR: Seven U.S. and 16 Japanese binucleate Rhizoctonia anastomosis tester isolates were characterized by restriction analysis of a ribosomal RNA (rRNA) gene by extracting genetic DNA from each isolate and amplified using the polymerase chain reaction.

Abstract: Seven U.S. and 16 Japanese binucleate Rhizoctonia anastomosis tester isolates, representing 21 different anastomosis groups, were characterized by restriction analysis of a ribosomal RNA (rRNA) gene. Genomic DNA was extracted from each isolate and a region of DNA coding for a portion of the 25S rRNA (rDNA) was amplified using the polymerase chain reaction. Five tester isolates (CAG1, AGF, AGI, AGJ, and AGK) produced either two or three bands ranging from 1.4 to 1.8 kilobases (kb), whereas five other tester isolates (CAG5, AGBa, AGC, AGD, and AGH) produced a single, 1.8-kb fragment (...)