Open AccessJournal Article
Chromosomal localization of the human placental lactogen-growth hormone gene cluster to 17q22-24.
TLDR
Recombinant plasmid HCS-pBR322 containing a 550-base-pair insert of cDNA to human placental lactogen mRNA was 3H-labeled by nick translation and hybridized in situ to human chromosome preparations in the presence of 10% dextran sulfate.Abstract:
Recombinant plasmid HCS-pBR322 containing a 550-base-pair (bp) insert of cDNA to human placental lactogen (hPL) mRNA was 3H-labeled by nick translation and hybridized in situ to human chromosome preparations in the presence of 10% dextran sulfate. A high percentage of cells (80%) were found to exhibit label on the distal end of the long arm of chromosome 17. Silver grains on this region constituted 25.5% of all labeled sites, allowing assignment of the hPL and growth hormone (hGH) genes, which have over 90% nucleotide homology in their coding sequences, to 17q22-24. A gene copy number experiment showed that both genes are present in approximately three copies per haploid genome.read more
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Protection against enteric salmonellosis in transgenic mice expressing a human intestinal defensin
TL;DR: Support is provided for a critical in vivo role of epithelial-derived defensins in mammalian host defence by showing that HD-5 expression in these mice is specific to Paneth cells and reflects endogenous enteric defensin gene expression.
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Genetic mapping of a gene causing hypertension in the stroke-prone spontaneously hypertensive rat.
Howard J. Jacob,Howard J. Jacob,Klaus Lindpaintner,Klaus Lindpaintner,Stephen E Lincoln,Kenro Kusumi,Ruth K. Bunker,Yipei Mao,Detlev Ganten,Victor J. Dzau,Eric S. Lander +10 more
TL;DR: Strong genetic evidence is reported that a gene, Bp1, having a major effect on blood pressure maps to rat chromosome 10 with a LOD score of 5.10 and is closely linked to the rat gene encoding angiotensin-converting enzyme (ACE), an enzyme that plays a major role in blood pressure homeostasis and is an important target of anti-hypertensive drugs.
Journal ArticleDOI
Absence of linkage between the angiotensin converting enzyme locus and human essential hypertension.
Xavier Jeunemaitre,Richard P. Lifton,Richard P. Lifton,Steven C. Hunt,Roger R. Williams,Jean Marc Lalouel +5 more
TL;DR: The localization of the ACE gene on the genetic map of chromosome 17 is reported, and an extremely polymorphic marker at the human growth hormone (hGH) locus which shows no recombination with ACE is identified which suggests that mutations at the ACE locus do not commonly contribute to the pathogenesis of hypertension.
Journal ArticleDOI
The roles of placental growth hormone and placental lactogen in the regulation of human fetal growth and development.
TL;DR: Taken together, studies of the hGH/hPL gene family during pregnancy reveal a complex interaction of the hormones with one another and with other growth factors.
Journal ArticleDOI
DNA probe localization at 18p113 band by in situ hybridization and identification of a small supernumerary chromosome
Marie Geneviève Mattei,Nicole Philip,E. Passage,Jean-Paul Moisan,Jean-Paul Moisan,Jean-Louis Mandel,Jean-Louis Mandel,Jean-François Mattei +7 more
TL;DR: Recombinant plasmid clone B74 containing a human single-copy DNA segment of 6 kilobases (kb) was localized by in situ hybridization on band p113 of chromosome 18 to identify precisely a small supernumerary chromosome as an isochromosome i(18p).
References
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The Isolation and Characterization of Linked δ- and β-Globin Genes from a Cloned Library of Human DNA
TL;DR: The two independently isolated β-globin clones differ from each other by the presence of a Pst I restriction enzyme cleavage site within the large intervening sequence of the δ- globin gene of one of the clones, which suggests that the human DNA carried in the two clones was derived from two homologous chromosomes which were heterozygous for the PstI restriction enzyme recognition sequence.
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Localization of single copy DNA sequences of G-banded human chromosomes by in situ hybridization.
Mary E. Harper,Grady F. Saunders +1 more
TL;DR: In situ hybridization to human metaphase chromosomes of the 3H-labeled cloned DNA resulted in highly significant labeling of band p36 of chromosome 1, such that 22% of all chromosome grains were located on this region.
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Three abundance classes in HeLa cell messenger RNA
TL;DR: Approximately 35,000 different poly(A)-containing RNA sequences are present in HeLa cell cytoplasm and the sequences are grouped in three distinct abundance classes.
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Stimulation by cyclic adenosine monophosphate of plasmid deoxyribonucleic acid replication and catabolite repression of the plasmid deoxyribonucleic acid-protein relaxation complex.
TL;DR: It was shown that synthesis of both the plasmid-determined protein colicin E1 and the protein component(s) of the ColE1 relaxation complex is mediated through the c-AMP-catabolite gene activator protein system.
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Sequences of Pituitary and Placental Lactogenic and Growth Hormones: Evolution from a Primordial Peptide by Gene Reduplication
TL;DR: The observation of internal sequence homologies within the pituitary growth hormone and prolactin and the placental lactogen molecules suggests that these polypeptide hormones may have evolved by genetic reduplication from a smaller common ancestral peptide.