Cloning and expression of a cDNA for mouse prostaglandin E receptor EP2 subtype.
Akiko Honda,Yukihiko Sugimoto,Tsunehisa Namba,Akiko Watabe,Atsushi Irie,Manabu Negishi,Shuh Narumiya,Atsushi Ichikawa +7 more
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TLDR
A functional cDNA clone encoding mouse EP2 subtype of prostaglandin (PG) E receptor was isolated from a mouse cDNA library by cross-hybridization with the mouse EP3 subtype PGE receptor cDNA, suggesting that this receptor is EP2Subtype.About:
This article is published in Journal of Biological Chemistry.The article was published on 1992-04-05 and is currently open access. It has received 531 citations till now. The article focuses on the topics: cDNA library & Complementary DNA.read more
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Prostanoid Receptors: Structures, Properties, and Functions
TL;DR: An overview of the current status of research on the prostanoid receptors is given and domains and amino acid residues conferring the specificities of ligand binding and signal transduction are being clarified.
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International Union of Pharmacology classification of prostanoid receptors: properties, distribution, and structure of the receptors and their subtypes.
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Regulation of Immune Responses by Prostaglandin E2
TL;DR: Targeting the production, degradation, and responsiveness to PGE2 provides tools to modulate the patterns of immunity in a wide range of diseases, from autoimmunity to cancer.
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Prostaglandin E Receptors
Yukihiko Sugimoto,Shuh Narumiya +1 more
TL;DR: Recent advances in PGE receptor research are reviewed, including studies on knock-out mice deficient in each EP subtype have defined PGE2 actions mediated by each subtype and identified the role eachEP subtype plays in various physiological and pathophysiological responses.
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Prostanoid receptors: subtypes and signaling.
TL;DR: Alternative splice variants described that alter the coding sequence in the C-terminal intracellular tail region modulate signal transduction, phosphorylation, and desensitization of these receptors, as well as altering agonist-independent constitutive activity.
References
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Single-step method of RNA isolation by acid guanidinium thiocyanate-phenol-chloroform extraction
TL;DR: A new method of total RNA isolation by a single extraction with an acid guanidinium thiocyanate-phenol-chloroform mixture is described, providing a pure preparation of undegraded RNA in high yield and can be completed within 4 h.
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A simple method for displaying the hydropathic character of a protein
Jack Kyte,Russell F. Doolittle +1 more
TL;DR: A computer program that progressively evaluates the hydrophilicity and hydrophobicity of a protein along its amino acid sequence has been devised and its simplicity and its graphic nature make it a very useful tool for the evaluation of protein structures.
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A new technique for the assay of infectivity of human adenovirus 5 DNA.
Frank L. Graham,A. J. Van Der Eb +1 more
TL;DR: A new technique for assaying infectivity of adenovirus 5 DNA has been developed and a reproducible relationship between amounts of DNA inoculated per culture and numbers of plaques produced was demonstrated.
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Lipofection: a highly efficient, lipid-mediated DNA-transfection procedure
Philip L. Felgner,Thomas R. Gadek,Marilyn Holm,Richard Bolton Roman,Hardy W. Chan,Michael Wenz,Jeffrey P. Northrop,Gordon M. Ringold,Mark Danielsen +8 more
TL;DR: Depending upon the cell line, lipofection is from 5- to greater than 100-fold more effective than either the calcium phosphate or the DEAE-dextran transfection technique.
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The scanning model for translation: an update.
TL;DR: The small (40S) subunit of eukaryotic ribosomes is believed to bind initially at the capped 5'-end of messenger RNA and then migrate, stopping at the first AUG codon in a favorable context for initiating translation.