Cloning of genes involved in membrane lipid synthesis. Effects of amplification of phosphatidylserine synthase in Escherichia coli.
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This article is published in Journal of Biological Chemistry.The article was published on 1981-03-10 and is currently open access. It has received 77 citations till now. The article focuses on the topics: Plasmid & Membrane lipids.read more
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Journal ArticleDOI
Linkage map of Escherichia coli K-12, edition 7.
TL;DR: This article corrects the article on p. 182 in vol.
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Bacterial lipids: Metabolism and membrane homeostasis
TL;DR: This review summarizes how the explosion of new information on the diversity of biochemical and genetic regulatory mechanisms has impacted the understanding of bacterial lipid homeostasis.
Journal ArticleDOI
Biosynthesis and function of phospholipids in Escherichia coli.
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Identification of a Non-mitochondrial Phosphatidylserine Decarboxylase Activity (PSD2) in the Yeast Saccharomyces cerevisiae
TL;DR: The present studies demonstrate the presence of a second enzyme activity (denoted PSD2) which, depending on the method of evaluation, accounts for 4-12% of the total cellular phosphatidylserine decarboxylase activity found in wild type.
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Phosphatidylserine decarboxylase from Saccharomyces cerevisiae. Isolation of mutants, cloning of the gene, and creation of a null allele.
TL;DR: Analysis of lipid synthesis and enzyme activity in null mutants indicates that there are two PSD genes, and Null mutations created by disrupting the PSD gene with TRP1 demonstrate that the gene is not essential for cell growth even when the engineered strains are deprived of choline and ethanolamine.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
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Construction and characterization of new cloning vehicles. II. A multipurpose cloning system.
Francisco Bolívar,Raymond L. Rodriguez,Patricia J. Greene,Mary C. Betlach,Herbert L. Heyneker,Herbert W. Boyer,Jorge H. Crosa,Stanley Falkow +7 more
TL;DR: In vitro recombination techniques were used to construct a new cloning vehicle, pBR322, which is a relaxed replicating plasmid, does not produce and is sensitive to colicin E1, and carries resistance genes to the antibiotics ampicillin (Ap) and tetracycline (Tc).
Journal ArticleDOI
A colony bank containing synthetic CoI EI hybrid plasmids representative of the entire E. coli genome
Louise Clarke,John Carbon +1 more
TL;DR: Over 80 hybrid Col El-DNA (E. coli), plasmid-bearing clones have been identified in the colony bank, and about 40 known E. coli genes have been tentatively assigned to these various plasmids.
Journal ArticleDOI
Nature of Col E 1 plasmid replication in Escherichia coli in the presence of the chloramphenicol.
TL;DR: The colicinogenic factor E(1) (Col E( 1)) in Escherichia coli continues to replicate by a semiconservative mechanism in the presence of chloramphenicol for 10 to 15 hr, long after chromosomal deoxyribonucleic acid (DNA) synthesis has terminated.
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Transformation of Salmonella typhimurium by Plasmid Deoxyribonucleic Acid
TL;DR: A modified transformation procedure that is effective for the introduction of plasmid deoxyribonucleic acid at high frequency into Salmonella typhimurium, as well as into Escherichia coli, is described.
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