Journal ArticleDOI
Comparative analysis of the expression of the extracellular matrix protein tenascin in normal human fetal, adult and tumor tissues.
Pier Giorgio Natali,Maria Rita Nicotra,Aldo Bigotti,Claudio Botti,Patrizia Castellani,Anna Maria Risso,Luciano Zardi +6 more
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TLDR
This study demonstrates that TN is detectable in embryonic and fetal tissues at least from the 10th week of gestation, and has the ability to accumulate in a highly variable manner in the ECM of tumors of the same and of different histotypes.Abstract:
Tenascin (TN) is a high-molecular-mass oligomeric glycoprotein of the extracellular matrix (ECM) endowed with a programmed expression in embryonic life and a neo-expression in the interstitium of some malignancies. Using monoclonal antibodies (MAbs) which identify human tenascin, we have conducted an extensive immunohistochemical analysis of TN expression in normal fetal and adult human tissues as well as in a wide variety of human tumors. Results of this study demonstrate that TN (1) is detectable in embryonic and fetal tissues at least from the 10th week of gestation; (2) is present in the interstitium of a variety of adult tissues of different embryonic origin; (3) may be neo-expressed in the stroma of benign and malignant tumors; (4) has the ability to accumulate in a highly variable manner in the ECM of tumors of the same and of different histotypes.read more
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Patent
Methods for targeting the vasculature of solid tumors
TL;DR: In this article, the authors present methods and compositions for targeting the vasculature of solid tumors using immunological and growth factor-based reagents, such as endoglin binding, or specific induction of endothelial cell surface antigens on vascular endothelial cells in solid tumors.
Journal ArticleDOI
Antibody-guided three-step therapy for high grade glioma with yttrium-90 biotin
Giovanni Paganelli,Chiara Maria Grana,Marco Chinol,Marta Cremonesi,Concetta De Cicco,Filippo de Braud,Chris Robertson,Stefano Zurrida,Chiara Casadio,Stefania Zoboli,Antonio G. Siccardi,Umberto Veronesi +11 more
TL;DR: An avidin-biotin pre-targeting approach for delivering therapeutic radionuclides to gliomas, using anti-tenascin monoclonal antibodies, which seems potentially effective for treating these tumours.
Patent
Compositions for targeting the vasculature of solid tumors
TL;DR: In this paper, the authors present methods and compositions for targeting the vasculature of solid tumors using immunological and growth factor-based reagents, such as endoglin binding, or specific induction of endothelial cell surface antigens on vascular endothelial cells in solid tumors.
Journal ArticleDOI
Design, construction, and characterization of a large synthetic human antibody phage display library.
Michaela Silacci,Simon Brack,Giulia Schirru,Jessica Mårlind,Anna Ettorre,Adrian Merlo,Francesca Viti,Dario Neri +7 more
TL;DR: The design, construction, characterization, and use of a large synthetic human antibody phage display library (ETH‐2‐Gold) containing three billion individual antibody clones, which exhibits efficient display of antibody fragments on filamentous phage, as assessed by immunoblot.
Journal ArticleDOI
Expression of different tenascin isoforms in normal, hyperplastic and neoplastic human breast tissues.
TL;DR: The data reported here suggest that, in the breast, expression of the high‐molecular‐mass TN isoform is a marker of stromal element proliferation and that thisTN isoform could play a role in generating a permissive environment for proliferation, invasion and metastasis of neoplastic epithelial cells.
References
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Journal ArticleDOI
Cleavage of Structural Proteins during the Assembly of the Head of Bacteriophage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products.
Journal Article
Cleavage of structural proteins during the assemble of the head of bacterio-phage T4
TL;DR: Using an improved method of gel electrophoresis, many hitherto unknown proteins have been found in bacteriophage T4 and some of these have been identified with specific gene products as mentioned in this paper.
Journal ArticleDOI
Electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets: procedure and some applications.
TL;DR: A method has been devised for the electrophoretic transfer of proteins from polyacrylamide gels to nitrocellulose sheets that results in quantitative transfer of ribosomal proteins from gels containing urea.
Journal ArticleDOI
Tumors: wounds that do not heal. Similarities between tumor stroma generation and wound healing.
TL;DR: Tumors of epithelioma are composed of two discrete but interdependent compartments: the malignant cells themselves and the stroma that they induce and in which they are dispersed.
Journal ArticleDOI
Tenascin: an extracellular matrix protein involved in tissue interactions during fetal development and oncogenesis
TL;DR: Of the extracellular matrix proteins compared, tenascin is the least effective substrate for attachment of primary mammary tumor cells, but the most effective in promoting cell growth after serum is removed from the culture medium.