Journal ArticleDOI
Concerted action of endogenous and heterologous phytase on phytic acid degradation in seed of transgenic wheat (Triticum aestivum L.).
TLDR
Metal-dye detection HPLC analysis of inositol phosphate degradation in flour from transgenic wheat materials possessing wheat endogenous 6-phytase and Aspergillus 3-phyTase activities is reported, finding that after 50 min incubation virtually all InsP5, InsP4 and InsP3 isomers are hydrolysed.Abstract:
Expression of heterologous phytases in crops offers a great potential for improving phosphate and mineral bioavailability in food and feed. In this context it is of relevance to describe the concerted action of endogenous and hetrologous phytases on the transgenic seed inositol phosphate profile. Here we report metal-dye detection HPLC analysis of inositol phosphate degradation in flour from transgenic wheat materials possessing wheat endogenous 6-phytase [EC 3.1.3.26] and Aspergillus 3-phytase [EC 3.1.3.8] activities under the control of the maize ubiquitin-1 promoter and the wheat high molecular weight glutenin subunit 1DX5 promoter respectively. During 50 min incubation there is an accumulation of InsP5 to InsP2 breakdown products in non-transgenic material. Aspergillus niger phytase specific breakdown products are transiently detected in transgenic material but after 50 min incubation virtually all InsP5, InsP4 and InsP3 isomers are hydrolysed.read more
Citations
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Journal ArticleDOI
Biofortifying crops with essential mineral elements
TL;DR: There is considerable genetic variation in crop species that can be harnessed for sustainable biofortification strategies, and new genotypes with higher mineral densities are being developed.
Journal ArticleDOI
Phytate: impact on environment and human nutrition. A challenge for molecular breeding
TL;DR: Biochemical data for purified and characterized phytases isolated from more than 23 plant species are presented, the dephosphorylation pathways of phytic acid by different classes ofphytases are compared, and the application of phytase in food and feed is discussed.
Journal ArticleDOI
Sowing the seeds of success: pharmaceutical proteins from plants.
TL;DR: The use of seeds for the production of pharmaceutical proteins, particularly replacement human proteins, recombinant antibodies and (oral) vaccines, has been explored, and the first commercial products have already reached the market.
Journal ArticleDOI
Endosperm-specific co-expression of recombinant soybean ferritin and Aspergillus phytase in maize results in significant increases in the levels of bioavailable iron.
Georgia Drakakaki,Sylvain Marcel,Raymond P. Glahn,Elizabeth K. Lund,Sandra Pariagh,Rainer Fischer,Paul Christou,Eva Stoger +7 more
TL;DR: The expression of recombinant ferritin and phytase could help to increase iron availability and enhance the absorption of iron, particularly in cereal-based diets that lack other nutritional components.
Journal ArticleDOI
Critical evaluation of strategies for mineral fortification of staple food crops.
Sonia Gómez-Galera,Eduard Rojas,Duraialagaraja Sudhakar,Changfu Zhu,A.M. Pelacho,Teresa Capell,Paul Christou,Paul Christou +7 more
TL;DR: A critical comparison of the strategies that have been developed to address deficiencies in five key mineral nutrients—iodine, iron, zinc, calcium and selenium is provided and the most recent advances in genetic engineering to increase mineral levels and bioavailability in the authors' most important staple food crops are discussed.
References
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Journal ArticleDOI
Phytic acid interactions in food systems
TL;DR: Development of methods for producing low-phytate food products must take into account the nature and extent of the interactions between phytic acid and other food components, such as pH-solubility profiles of the proteins and the cookability of the seeds.
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Distribution of phytate and nutritionally important elements among the morphological components of cereal grains
Journal ArticleDOI
Structure and transcription of the nopaline synthase gene region of T-DNA.
TL;DR: The strategy used to determine the sequence produced two opposing series of defined, asymmetric deletions across the target DNA region, some of which may serve future purposes in the exploitation of this sequence, which is known to be expressed in a wide variety of host plant tissues.
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Genetic engineering approaches to improve the bioavailability and the level of iron in rice grains
TL;DR: High phytase rice, with an increased iron content and rich in cysteine-peptide, has the potential to greatly improve iron nutrition in rice-eating populations.
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Engineering crop plants: getting a handle on phosphate
TL;DR: Engineering crop plants to produce a heterologous phytase improves phosphate bioavailability and reduces phytic acid excretion, which reduces the phosphate load on agricultural ecosystems and thereby alleviates eutrophication of the aquatic environment.