Journal ArticleDOI
Conformation and thermostability of oligonucleotide d(GGTTGGTGTGGTTGG) containing thiophosphoryl internucleotide bonds at different positions.
M. A. Zaitseva,Dmitry N. Kaluzhny,Anna K. Shchyolkina,Olga F. Borisova,Igor P. Smirnov,Galina E. Pozmogova +5 more
TLDR
According to the thrombin time test, the aptamer with thio-modifications in both TT loops (LL11) exhibits the same antithrombin efficiency as the original TBA, and shows better stability against DNA nuclease compared to that of TBA.About:
This article is published in Biophysical Chemistry.The article was published on 2010-01-01. It has received 53 citations till now. The article focuses on the topics: Aptamer.read more
Citations
More filters
Journal ArticleDOI
The application of DNA and RNA G-quadruplexes to therapeutic medicines
TL;DR: A critical review of the important underlying characteristics of G-quadruplexes that make them functional, stable, and predictable nanoscaffolds and a critical evaluation of the application of high-resolution structural biology and its ability to provide meaningful and valid models for future applications.
Journal ArticleDOI
High-resolution structures of two complexes between thrombin and thrombin-binding aptamer shed light on the role of cations in the aptamer inhibitory activity
Irene Russo Krauss,Antonello Merlino,Antonio Randazzo,Ettore Novellino,Lelio Mazzarella,Filomena Sica +5 more
TL;DR: A high-resolution crystallographic structural characterization of the thrombin–TBA complex formed in the presence of Na+ or K+ and a circular dichroism study of the structural stability of the aptamer both free and complexed with α-thrombin are presented, showing different effects exerted by Na+ and K+ on the inhibitory activity of TBA.
Journal ArticleDOI
Chemical Modifications of Nucleic Acid Aptamers for Therapeutic Purposes.
TL;DR: This review will discuss methods to conquer challenges of susceptibility to nuclease degradation, rapid excretion through renal filtration and insufficient binding affinity, and highlight recent developments of chemical modifications and technological advances that may enable early aptamers to be translated into clinical therapeutics.
Journal ArticleDOI
Aptamers Chemistry: Chemical Modifications and Conjugation Strategies.
Fadwa Odeh,Hamdi Nsairat,Walhan Alshaer,Mohammad A. Ismail,Ezaldeen Esawi,Bara’a M. Qaqish,Abeer Al Bawab,Said I. Ismail,Said I. Ismail +8 more
TL;DR: Aptamers are short single-stranded RNA or DNA oligonucleotides capable of folding into complex 3D structures, enabling them to bind to a large variety of targets ranging from small ions to an entire organism, and they are superior regarding a longer shelf life, simple production and chemical modification.
References
More filters
Journal ArticleDOI
Selection of single-stranded DNA molecules that bind and inhibit human thrombin.
TL;DR: The isolation of single-stranded DNA aptamers to the protease thrombin of the blood coagulation cascade is described and binding affinities in the range 25–200 nM are reported.
Journal ArticleDOI
OligoCalc: an online oligonucleotide properties calculator.
TL;DR: OligoCalc incorporates three common methods for calculating oligonucleotide-melting temperatures, including a nearest-neighbor thermodynamic model for melting temperature.
Journal ArticleDOI
Calculating thermodynamic data for transitions of any molecularity from equilibrium melting curves
TL;DR: The general equations and methods of analysis described in this paper should be of particular interest to laboratories that currently are investigating association and dissociation processes in nucleic acids that exhibit molecularities greater than two.
Journal ArticleDOI
Thrombin-binding DNA aptamer forms a unimolecular quadruplex structure in solution.
TL;DR: This aptamer structure was determined by NMR and illustrates that this molecule forms a specific folded structure, which may be used in the further development of oligonucleotide-based thrombin inhibitors.