Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.
Jeffrey E. Berman,S. J. Mellis,Roberta R. Pollock,Cassandra L. Smith,Heikyung Suh,B. Heinke,C. Kowal,Urvashi Surti,Leonard Chess,C. R. Cantor +9 more
TLDR
The first report of the physical linkage of the variable and constant loci of a human Ig gene family is provided by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.Abstract:
We present a detailed analysis of the content and organization of the human immunoglobulin VH locus. Human VH genes representing five distinct families were isolated, including novel members belonging to two out of three of the known VH gene families (VH1 and VH3) as well as members of three new families (VH4, VH5, and VH6). We report the nucleotide sequence of 21 novel human VH genes, many of which belong to the three new VH gene families. In addition, we provide a preliminary analysis of the organization of these gene segments over the full extent of the locus. We find that the five multi-segment families (VH1-5) have members interspersed over nearly the full 1500-2000 kb of the VH locus, and estimate that the entire heavy chain locus covers 2500 kb or less. Finally, we provide the first report of the physical linkage of the variable and constant loci of a human Ig gene family by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.read more
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Journal ArticleDOI
A linkage map of the mouse immunoglobulin lambda light chain locus.
TL;DR: The mouse immunoglobulin lambda light chain locus has been linked using field inversion gel electrophoresis and the gap between the V and J-C gene segments in the lambda 1 gene cluster is mapped to approximately 70 kb, further evidence that duplication of an ancestral locus occurred.
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A chromosomal basis of lymphoid malignancy in man.
T Boehm,Terence H. Rabbitts +1 more
TL;DR: This process of DNA rearrangement also allows errors to occur, which include aberrant intra-gene rearrangements and in addition the creation of chromosomal abnormalities which may involve proto-oncogene activation resulting in appearance of lymphoid tumours.
Patent
High affinity human antibodies and human antibodies against human antigens
TL;DR: In this paper, a transgenic non-human animal capable of producing high affinity human sequence antibodies was proposed, which was also directed to human sequence antibody specific for human antigens, such as human CD4.
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Immunoglobulin heavy chain loci of mouse and human
Tasuku Honjo,Fumihiko Matsuda +1 more
TL;DR: This chapter summarizes the current knowledge of the organization and structure of the mouse and human immunoglobulin (IgH) loci and to discuss its biological significance and implications.
Journal ArticleDOI
Restricted diversity of the variable region nucleotide sequences of the heavy and light chains of a human rheumatoid factor
TL;DR: The results suggest that variable region genes of RFs are highly conserved and that YES8c VH, as well as V kappa, may be identical to heavy and light chains expressed during early B cell development.