Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.
Jeffrey E. Berman,S. J. Mellis,Roberta R. Pollock,Cassandra L. Smith,Heikyung Suh,B. Heinke,C. Kowal,Urvashi Surti,Leonard Chess,C. R. Cantor +9 more
TLDR
The first report of the physical linkage of the variable and constant loci of a human Ig gene family is provided by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.Abstract:
We present a detailed analysis of the content and organization of the human immunoglobulin VH locus. Human VH genes representing five distinct families were isolated, including novel members belonging to two out of three of the known VH gene families (VH1 and VH3) as well as members of three new families (VH4, VH5, and VH6). We report the nucleotide sequence of 21 novel human VH genes, many of which belong to the three new VH gene families. In addition, we provide a preliminary analysis of the organization of these gene segments over the full extent of the locus. We find that the five multi-segment families (VH1-5) have members interspersed over nearly the full 1500-2000 kb of the VH locus, and estimate that the entire heavy chain locus covers 2500 kb or less. Finally, we provide the first report of the physical linkage of the variable and constant loci of a human Ig gene family by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.read more
Citations
More filters
Journal ArticleDOI
CD5+ B cells and the immune system.
TL;DR: In both mice and men there is evidence in favour of CD5 being an activation antigen rather than a lineage marker, but the jury is still out! the frequency ofCD5+ B cells appears to be under genetic influence.
Journal ArticleDOI
Organization of the Ig VH locus in mice and humans.
TL;DR: The potential influence of gene organization on the activation of the VH gene repertoire during the development of the mammalian immune system is discussed.
Journal ArticleDOI
Physical linkage of a variable region segment and the joining region segment of the human immunoglobulin heavy chain locus.
TL;DR: The results indicate that the Vh-VI gene is the most proximal VH segments among the known VH family members and that the distance between the VH and JH segments is not longer than 70 kb in the human genome.
Journal ArticleDOI
Diversity of immunoglobulin heavy chain gene segment rearrangement in B lymphoblastoid cell lines from X-linked agammaglobulinemia patients.
Erik Timmers,Marcel J. H. Kenter,Allan Thompson,Margriet E. M. Kraakman,Jeffrey E. Berman,Frederick W. Alt,Ruud K. B. Schuurman +6 more
TL;DR: The results supported the conclusion that the few B lymphocytes in peripheral blood of XLA patients exhibit all mechanisms that generate immunoglobulin (Ig) heavy (H) chain diversity, and unique features of the recombined and expressed VHDJH regions in XLA may explain the inability of X LA patients to respond to a variety of antigens.
Patent
Detection of minimal residual disease in lymphoid malignancies
TL;DR: In this article, a method for detection of minimal residual disease in lymphoid malignancies by the following steps: (i) amplifying DNA from an original tumor using universal J and V primers in a polymerase chain reaction; (ii) preparing a single stranded probe from a discrete fragment of the amplification product of step (i), and (iii) amplify DNA from a test specimen suspected of containing residual disease with the same J/V primers used in step (ii), subjecting the hybridization product to S1 nuclease digestion; and (vi) determining the