Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.
Jeffrey E. Berman,S. J. Mellis,Roberta R. Pollock,Cassandra L. Smith,Heikyung Suh,B. Heinke,C. Kowal,Urvashi Surti,Leonard Chess,C. R. Cantor +9 more
TLDR
The first report of the physical linkage of the variable and constant loci of a human Ig gene family is provided by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.Abstract:
We present a detailed analysis of the content and organization of the human immunoglobulin VH locus. Human VH genes representing five distinct families were isolated, including novel members belonging to two out of three of the known VH gene families (VH1 and VH3) as well as members of three new families (VH4, VH5, and VH6). We report the nucleotide sequence of 21 novel human VH genes, many of which belong to the three new VH gene families. In addition, we provide a preliminary analysis of the organization of these gene segments over the full extent of the locus. We find that the five multi-segment families (VH1-5) have members interspersed over nearly the full 1500-2000 kb of the VH locus, and estimate that the entire heavy chain locus covers 2500 kb or less. Finally, we provide the first report of the physical linkage of the variable and constant loci of a human Ig gene family by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.read more
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Complete sequence of the genes encoding the VH and VL regions of low- and high- affinity monoclonal lgM and lgA1 rheumatoid factors produced by CD5+ B cells from a rheumatoid arthritis patient
Nagaradona Harindranath,Inna Goldfarb,Hideyuki Ikematsu,Samuele E. Burastero,Ronald L. Wilder,Abner Louis Notkins,Paolo Casali +6 more
TL;DR: Sequencing of the genes encoding the H chain CDR3 (D segments) revealed that all three low-affinity polyreactive RF mAb displayed a much longer D segment than their high-Affinity monoreactive counterparts, raising the possibility that a long D segment may be one of the factors involved in antibody polyreactivity.
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Restricted immunoglobulin VH usage and VDJ combinations in the human response to Haemophilus influenzae type b capsular polysaccharide. Nucleotide sequences of monospecific anti-Haemophilus antibodies and polyspecific antibodies cross-reacting with self antigens.
Elisabeth E. Adderson,Penelope G. Shackelford,A Quinn,P M Wilson,Madeleine W. Cunningham,Richard A. Insel,William L. Carroll +6 more
TL;DR: Significant restriction in VH usage and VDJ recombination in the anti-Hib PS response is demonstrated and it is confirmed that autoantibodies may be elicited during normal immune responses.
Journal ArticleDOI
Distinct ongoing Ig heavy chain rearrangement processes in childhood B-precursor acute lymphoblastic leukemia.
E. J. Steenbergen,O. J. H. M. Verhagen,EF van Leeuwen,A. E. G. K. Von Dem Borne,C. E. Van Der Schoot +4 more
TL;DR: The findings suggest that the pattern of ongoing rearrangements in an individual patient reflects the IgH rearrangement status of the precursor cell at the time of malignant transformation and may allow a prediction of the reliability of complementarity determining region 3 probes for the detection of minimal residual disease.
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Immunoglobulin V regions and the B cell.
A. K. Stewart,Robert S. Schwartz +1 more
TL;DR: There is now substantial evidence that a small group of V genes predominates in the Ig repertoire of preimmune B cells, and these genes may reflect preferential accessibility to recombinase, homology-directed V gene rearrangement, promoters and enhances of V gene transcription, or positive and negative selection mediated by the anti-self binding properties of the B cells surface Ig.
Journal ArticleDOI
Physical map of the 3' region of the human immunoglobulin heavy chain locus: clustering of autoantibody-related variable segments in one haplotype.
Euy Kyun Shin,Fumihiko Matsuda,Hitoshi Nagaoka,Yosho Fukita,Takashi Imai,Kazushige Yokoyama,Eiichi Soeda,Tasuku Honjo +7 more
TL;DR: The results suggest that VH haplotypes may have linkage with autoantibody production, and the physical map of the 3′ region of the human immunoglobulin heavy chain variable region (VH) genes is constructed.