Content and organization of the human Ig VH locus: definition of three new VH families and linkage to the Ig CH locus.
Jeffrey E. Berman,S. J. Mellis,Roberta R. Pollock,Cassandra L. Smith,Heikyung Suh,B. Heinke,C. Kowal,Urvashi Surti,Leonard Chess,C. R. Cantor +9 more
TLDR
The first report of the physical linkage of the variable and constant loci of a human Ig gene family is provided by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.Abstract:
We present a detailed analysis of the content and organization of the human immunoglobulin VH locus. Human VH genes representing five distinct families were isolated, including novel members belonging to two out of three of the known VH gene families (VH1 and VH3) as well as members of three new families (VH4, VH5, and VH6). We report the nucleotide sequence of 21 novel human VH genes, many of which belong to the three new VH gene families. In addition, we provide a preliminary analysis of the organization of these gene segments over the full extent of the locus. We find that the five multi-segment families (VH1-5) have members interspersed over nearly the full 1500-2000 kb of the VH locus, and estimate that the entire heavy chain locus covers 2500 kb or less. Finally, we provide the first report of the physical linkage of the variable and constant loci of a human Ig gene family by demonstrating that the most proximal known human VH segments lie within 100 kb of the constant region locus.read more
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Molecular analysis of human immunoglobulin Vλ germline genes: Subgroups VλIII and VλIV
M.Denise Daley,Peng Hong-qi,Virginia Misener,Liu Xiao-Yuan,Pojen P. Chen,Katherine A. Siminovitch +5 more
TL;DR: Three human immunoglobulin Vλ germline genes have been isolated and one of the VλIV genes appear to be functional, despite deviations from the reported consensus sequences in their promoter TATA-box and recombination signal sequence elements.
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Idiotypic cross-reactivity of immunoglobulins expressed in Waldenström's macroglobulinemia, chronic lymphocytic leukemia, and mantle zone lymphocytes of secondary B-cell follicles.
TL;DR: It is found that such anti-CRI MoAbs stain a subpopulation of cells within the mantle zones surrounding the germinal centers of normal human tonsil and share idiotypic determinants with Igs expressed in B-cell CLL (B-CLL) and Waldenstrom's macroglobulinemia.
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Molecular characterization of a human anti-Rh(D) antibody with a DH segment encoded by a germ-line sequence.
TL;DR: In this paper, an anti-Rh(D)mAb λ-chain variable region with those of the other available human λ chains revealed that it belonged to the human VλI (Vλ, variable region of λ chain) subgroup.
Journal ArticleDOI
Human splenic IgM immunoglobulin transcripts are mutated at high frequency
TL;DR: Human spleen immunoglobulin gene rearrangements that used the VH6 gene and were expressed with IgM were characterized for their frequency of somatic hypermutation and the possibility that IgM-expressing B cells may serve as human memory B cells is supported.
Journal ArticleDOI
Modulation and high frequency expression of autoantibody-associated cross-reactive idiotypes linked to the VHI subgroup in CD5-expressing B lymphocytes from patients with chronic lymphocytic leukaemia (B-CLL).
TL;DR: An over‐expression of VHI and VKIII gene products in B‐CLL is demonstrated and it is suggested that B cells expressing these CRI are particularly susceptible to lymphoproliferative stimuli.