Control of cell length in Bacillus subtilis.
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TLDR
During inhibition of deoxyribonucleic acid synthesis in Bacillus subtilis 168 Thy-minus Tryp-minus, the rate of length extension is constant and the ratio of average cell length to numbers of nuclei per cell in exponential cultures is constant over a fourfold range of growth rates.Abstract:
During inhibition of deoxyribonucleic acid synthesis in Bacillus subtilis 168 Thy-minus Tryp-minus, the rate of length extension is constant. A nutritional shift-up during thymine starvation causes an acceleration in the linear rate of length extension. During a nutritional shift-up in the presence of thymine, the rate of length extension gradually increases, reaching a new steady state at about 50 min before the new steady-state rate of cell division is reached. The steady-state rates of nuclear division and length extension are reached at approximately the same time. The ratio of average cell length to numbers of nuclei per cell in exponential cultures is constant over a fourfold range of growth rates. These observations are consistent with: (i) surface growth zones which operate at a constant rate of length extension under any one growth condition, but which operate at an absolute rate proportional to the growth rate of the culture, (ii) a doubling in number of growth zones at nuclear segregation, and (iii) a requirement for deoxyribonucleic acid replication for the doubling in a number of sites.read more
Citations
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Frequency of Dividing Cells, a New Approach to the Determination of Bacterial Growth Rates in Aquatic Environments
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Chromosome partitioning in Escherichia coli: novel mutants producing anucleate cells.
TL;DR: Results suggest that the mutant is defective in the chromosome positioning at regular intracellular positions and fails frequently to partition the replicated daughter chromosomes into both daughter cells, resulting in production of one anucleate daughter cell and one with two chromosomes.
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Fractionated phytoplankton primary production, exudate release and bacterial production in a Baltic eutrophication gradient
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TL;DR: It was demonstrated that selective filtration does not quantitatively separate photoautotrophs and bacteria in the northern Baltic proper and in the eutrophicated area.
Chromosome Partitioning inEscherichia coli: NovelMutants Producing Anucleate Cells
TL;DR: In this article, the authors have isolated a type of Escherichia coli mutants which formed anucleate cells, named mukA1, by using newly developed techniques and found that the mutant is defective in the chromosome positioning at regular intracellular positions and fails frequently to partition the replicated daughter chromosomes into both daughter cells.
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A Metabolic Sensor Governing Cell Size in Bacteria
Richard B. Weart,Amy S. Lee,An-Chun Chien,Daniel P. Haeusser,Norbert S. Hill,Petra Anne Levin +5 more
TL;DR: The identification of a metabolic sensor that couples nutritional availability to division in Bacillus subtilis is reported, which serves to maintain a constant ratio of FtsZ rings to cell length regardless of growth rate and ensures that cells reach the appropriate mass and complete chromosome segregation prior to cytokinesis.
References
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On the Regulation of DNA Replication in Bacteria
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Dependency on Medium and Temperature of Cell Size and Chemical Composition during Balanced Growth of Salmonella typhimurium
TL;DR: Cell mass, the average number of nuclei/cell and the content of RNA and DNA were studied in Salmonella typhimurium during balanced (steady state) growth in different media, indicating that this organism exists in one of a large number of possible stable physiological states.
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Growth of the Bacterial Cell
William D. Donachie,Ken Begg +1 more
TL;DR: Cell growth and division, and the way in which chromosome segregation is coordinated with these events, are discussed in terms of a model based on the concept of a unit cell—the smallest viable bacterium.
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Growth Rate and Generation Time of Bacteria, with Special Reference to Continuous Culture
TL;DR: The relations between growth rate, generation time distribution and age distribution in growing bacterial cultures are derived and it is concluded that the effect of inheritance on generation time is probably negligible.
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The transition between different physiological states during balanced growth of Salmonella typhimurium.
TL;DR: When cultures of Salmonella typhimurium undergoing balanced growth are shifted from one medium to another, a definite pattern of rate changes is observed and rate maintenance effect on DNA synthesis and cell division is discussed in terms of specific rate-controlling mechanisms.