Correlation of Bacterial Lipid Composition with Antibiotic Resistance
Reads0
Chats0
TLDR
As significant alterations of fatty acid composition were noted in gram-negative strains resistant to antibiotics, it is suggested that resistance is correlated to membrane or envelope lipid composition.Abstract:
The correlation of bacterial lipid composition with antibiotic resistance was investigated with particular emphasis on those organisms in which resistance may be related to membrane or envelope structure or function, as in resistance to tetracyclines and polymyxin. Chloroform-methanol-extractable lipids, phosphatidyl ethanolamine fractions, and both fatty acids of these lipid fractions and total fatty acids were studied by using thin-layer chromatography, gas chromatography, and infrared spectroscopy. Consistent quantitative differences were found between the fatty acid compositions of sensitive and resistant strains. Most notable was the fact that, in gram-negative organisms, resistant strains showed decreases in cyclopropane acids as compared with sensitive strains. These changes were found to be inherent in the strains and not due to growth stage or culture age. No significant qualitative differences were noted. In contrast, no such variation in fatty acid content was observed in penicillin-sensitive and resistant strains of gram-positive cocci. As significant alterations of fatty acid composition were noted in gram-negative strains resistant to antibiotics, we suggest that resistance is correlated to membrane or envelope lipid composition.read more
Citations
More filters
Journal ArticleDOI
Resistance of Pseudomonas to Quaternary Ammonium Compounds: II. Cross-Resistance Characteristics of a Mutant of Pseudomonas aeruginosa
TL;DR: Tube dilution experiments showed that benzalkonium chloride (BC)-resistant mutants of Pseudomonas aeruginosa grown in the presence of 1,000 mug of BC per ml were at least 20 times more sensitive to polymyxin B and colistin sulfate than the BC-sensitive (BCS) parent strain.
Book ChapterDOI
Walls and Membranes in Bacteria
D.A. Reaveley,R.E. Burge +1 more
TL;DR: This chapter describes the intracytoplasmic and cytop lasmic membranes of gram positive and negative bacteria along with details about bacterial cell wall.
Book ChapterDOI
Mode of Action of the Tetracyclines and the Nature of Bacterial Resistance to Them
TL;DR: In an introductory chapter to a symposium on biochemical studies of antimicrobial drugs, Gale (1966) suggests that advances in the development or use of a particular antibiotic will depend upon answers to at least five questions.
Journal ArticleDOI
Comprehensive analysis of phospholipids and glycolipids in the opportunistic pathogen Enterococcus faecalis
Rafi Rashid,Amaury Cazenave-Gassiot,Iris H. Gao,Zeus J. Nair,Jaspal Kaur Kumar,Liang Gao,Kimberly A. Kline,Markus R. Wenk +7 more
TL;DR: This lipidome map is the first comprehensive analysis of membrane phospholipids and glycolipids in the important human pathogen E. faecalis, for which antimicrobial resistance and altered lipid homeostasis have been intimately linked.
Journal ArticleDOI
Studies on the antibacterial activity of dodecylglycerol. Its limited metabolism and inhibition of glycerolipid and lipoteichoic acid biosynthesis in Streptococcus mutans BHT
TL;DR: Growth-inhibitory concentrations of racemic sn-1(3)-dodecylglycerol inhibit the incorporation of [14C] glycerol into lipids and lipoteichoic acid of Streptococcus mutans BHT and alter the per cent composition of the Glycerolipids.
References
More filters
Journal ArticleDOI
A simple method for the isolation and purification of total lipides from animal tissues.
TL;DR: In this paper, the authors described a simplified version of the method and reported the results of a study of its application to different tissues, including the efficiency of the washing procedure in terms of the removal from tissue lipides of some non-lipide substances of special biochemical interest.
Book
The infra-red spectra of complex molecules
TL;DR: In this article, the authors present a survey of research work in physics, physical sciences, and physical chemistry, focusing on physics, chemistry, physics, and biology. But they do not discuss their work in this paper.
Journal ArticleDOI
Mutants of escherichia coli requiring methionine or vitamin b12
TL;DR: Certain biochemical properties of auxotrophicl mutants of Escherichia coli with specific growth requirements for most of the known water-soluble vitamins, as well as of others responding to methionine but not to B,2 are described.
Journal ArticleDOI
A simple, specific spray for the detection of phospholipids on thin-layer chromatograms.
John C. Dittmer,Robert L. Lester +1 more
TL;DR: A chemical reagent spray specific for phosphate esters, based on molybdenum blue, gives an instantaneous, specific reaction with phospholipids on silica gel or alumina thin layer chromatography (TLC) plates.
Related Papers (5)
A simple method for the isolation and purification of total lipides from animal tissues.
Lipid alterations in cell envelopes of polymyxin-resistant Pseudomonas aeruginosa isolates.
R S Conrad,H E Gilleland +1 more