Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å.
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TLDR
The crystal structure of photosystem II is reported, finding that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn4CaO5 cluster; some of them may therefore serve as substrates for dioxygen formation.Abstract:
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail.read more
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Journal ArticleDOI
Oxygen-evolving Mn cluster in photosystem II: the protonation pattern and oxidation state in the high-resolution crystal structure.
TL;DR: It is shown that the experimental structure is a mixture of states containing highly reduced forms, with the largest contribution (almost 60%) from the S(-3)-state, Mn(II,II,III, III,III).
Journal ArticleDOI
Light-dependent chlorophyll f synthase is a highly divergent paralog of PsbA of photosystem II
TL;DR: Cyanobacteria that are able to use FRL for photosynthesis have evolved a novel far-red light photoacclimation (FaRLiP) mechanism to gain a strong selective advantage over other cyanobacteria.
Journal ArticleDOI
Light-harvesting complex II (LHCII) and its supramolecular organization in Chlamydomonas reinhardtii
Bartlomiej Drop,Mariam T. Webber-Birungi,K.N. Sathish Yadav,Alicja Filipowicz-Szymanska,Fabrizia Fusetti,Egbert J. Boekema,Roberta Croce +6 more
TL;DR: The data indicate that there are at least six LHCII trimers per dimeric core in the thylakoid membranes, meaning that the antenna size of PSII of C. reinhardtii is larger than that of plants.
Journal ArticleDOI
Mechanism of light induced water splitting in Photosystem II of oxygen evolving photosynthetic organisms.
TL;DR: The protein matrix of the WOC and bound water molecules provide an optimized dynamic landscape of hydrogen bonded protons for catalyzing oxidative water splitting energetically driven by light induced formation of the cation radical P680(+·) and the PS II core acts as a molecular machine formed during a long evolutionary process.
Journal ArticleDOI
Photosystem II, a growing complex : Updates on newly discovered components and low molecular mass proteins
TL;DR: Interestingly it is found that the chloroplast-localized genes encoding PSII proteins display a different response to environmental and stress conditions compared to the nuclear localized genes.
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