Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å.
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The crystal structure of photosystem II is reported, finding that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn4CaO5 cluster; some of them may therefore serve as substrates for dioxygen formation.Abstract:
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail.read more
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Journal ArticleDOI
The nature of chemical bonds of the CaMn4O5 cluster in oxygen evolving complex of photosystem II: Jahn‐Teller distortion and its suppression by Ca doping in cubane structures
Kizashi Yamaguchi,Shusuke Yamanaka,Hiroshi Isobe,T. Saito,K. Kanda,Yasufumi Umena,Keisuke Kawakami,Jian Ren Shen,N. Kamiya,Mitsutaka Okumura,Hironobu Nakamura,Mitsuo Shoji,Yasunori Yoshioka +12 more
TL;DR: In this article, full geometry optimizations of mixed-valence (MV) cubane Mn(III)4-ωMn(IV)ωO4 cluster (ω = 0-4) by UB3LYP have been performed to elucidate Jahn-Teller (JT) effects for the Mn(II) ions.
Journal ArticleDOI
Photosynthetic water oxidation: binding and activation of substrate waters for O–O bond formation
TL;DR: A detailed analysis of S-state dependent substrate water binding kinetics taking into consideration data from Mn coordination complexes supports a model in which the substrate waters are both bound as terminal ligands and react via a water-nucleophile attack mechanism.
Journal ArticleDOI
N-formylkynurenine as a marker of high light stress in photosynthesis.
TL;DR: The results are consistent with a role for the CP43 NFK modification in photoinhibition, the product formed from reaction of tryptophan with singlet oxygen, which can be produced under high light stress in PSII.
Journal ArticleDOI
The First State in the Catalytic Cycle of the Water-Oxidizing Enzyme: Identification of a Water-Derived μ-Hydroxo Bridge
Thomas Lohmiller,Vera Krewald,Arezki Sedoud,A. William Rutherford,Frank Neese,Wolfgang Lubitz,Dimitrios A. Pantazis,Nicholas Cox,Nicholas Cox +8 more
TL;DR: A structural model for the S0 state is proposed that is consistent with available experimental data and explains the observed evolution of water exchange kinetics in the first three states of the catalytic cycle.
Journal ArticleDOI
Nanoscale manganese oxide within Faujasite zeolite as an efficient and biomimetic water oxidizing catalyst
TL;DR: Nanoscale manganese oxides within Faujasite zeolite have been synthesized with a simple method and showed efficient water oxidizing activity in the presence of cerium(IV) ammonium nitrate as a non-oxo transfer oxidant.
References
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Book ChapterDOI
Processing of X-ray diffraction data collected in oscillation mode
Zbyszek Otwinowski,Wladek Minor +1 more
TL;DR: The methods presented in the chapter have been applied to solve a large variety of problems, from inorganic molecules with 5 A unit cell to rotavirus of 700 A diameters crystallized in 700 × 1000 × 1400 A cell.
Journal ArticleDOI
Features and development of Coot.
TL;DR: Coot is a molecular-graphics program designed to assist in the building of protein and other macromolecular models and the current state of development and available features are presented.
Journal ArticleDOI
The CCP4 suite: programs for protein crystallography
TL;DR: The CCP4 (Collaborative Computational Project, number 4) program suite is a collection of programs and associated data and subroutine libraries which can be used for macromolecular structure determination by X-ray crystallography.
Journal ArticleDOI
Crystallography & NMR System: A New Software Suite for Macromolecular Structure Determination
Axel T. Brunger,Axel T. Brunger,Paul D. Adams,G M Clore,W. L. DeLano,Piet Gros,R.W. Grosse-Kunstleve,R.W. Grosse-Kunstleve,Jiansheng Jiang,J. Kuszewski,Michael Nilges,Navraj S. Pannu,Randy J. Read,Luke M. Rice,Thomas Simonson,Gregory L. Warren +15 more
TL;DR: The Crystallography & NMR System (CNS) as mentioned in this paper is a software suite for macromolecular structure determination by X-ray crystallography or solution nuclear magnetic resonance (NMR) spectroscopy.
Journal ArticleDOI
Automatic processing of rotation diffraction data from crystals of initially unknown symmetry and cell constants
TL;DR: Kabsch et al. as discussed by the authors developed an algorithm for the automatic interpretation of a given set of observed reciprocal-lattice points by extracting a reduced cell and assigning indices to each reflection by a graph-theoretical implementation of the local indexing method.