Crystal structure of oxygen-evolving photosystem II at a resolution of 1.9 Å.
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The crystal structure of photosystem II is reported, finding that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn4CaO5 cluster; some of them may therefore serve as substrates for dioxygen formation.Abstract:
Photosystem II is the site of photosynthetic water oxidation and contains 20 subunits with a total molecular mass of 350 kDa. The structure of photosystem II has been reported at resolutions from 3.8 to 2.9 angstrom. These resolutions have provided much information on the arrangement of protein subunits and cofactors but are insufficient to reveal the detailed structure of the catalytic centre of water splitting. Here we report the crystal structure of photosystem II at a resolution of 1.9 angstrom. From our electron density map, we located all of the metal atoms of the Mn(4)CaO(5) cluster, together with all of their ligands. We found that five oxygen atoms served as oxo bridges linking the five metal atoms, and that four water molecules were bound to the Mn(4)CaO(5) cluster; some of them may therefore serve as substrates for dioxygen formation. We identified more than 1,300 water molecules in each photosystem II monomer. Some of them formed extensive hydrogen-bonding networks that may serve as channels for protons, water or oxygen molecules. The determination of the high-resolution structure of photosystem II will allow us to analyse and understand its functions in great detail.read more
Citations
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Journal ArticleDOI
Limitations to photosynthesis by proton motive force-induced photosystem II photodamage
Geoffry A. Davis,Atsuko Kanazawa,Mark Aurel Schöttler,Kaori Kohzuma,John E. Froehlich,A. William Rutherford,Mio Satoh-Cruz,Deepika Minhas,Stefanie Tietz,Amit Dhingra,David Kramer +10 more
TL;DR: The effects of elevated pmf on photosynthesis in a library of Arabidopsis thaliana mutants with altered rates of thylakoid lumen proton efflux are seen, suggesting that Δψ-induced photodamage represents a previously unrecognized limiting factor for plant productivity under dynamic environmental conditions seen in the field.
Journal ArticleDOI
Primary electron transfer processes in photosynthetic reaction centers from oxygenic organisms.
Mahir D. Mamedov,Govindjee,Victor A. Nadtochenko,Victor A. Nadtochenko,Alexey Yu. Semenov,Alexey Yu. Semenov +5 more
TL;DR: The results suggest that the sequence of the primary electron transfer reactions is P680-→ ChlD1 → PheD1–→ QA →-QA (PS II) and P700-→-A0A/A0B →–A1 a/A1B (PS I), however, alternate routes of charge separation in PS II, under different excitation conditions, are not ruled out.
Journal ArticleDOI
Substrate Water Exchange for the Oxygen Evolving Complex in PSII in the S1, S2, and S3 States
TL;DR: Three S-states have been studied and the rates have been well reproduced by the calculations, and the surprising experimental finding that water exchange in S1 is slower than the one in S2 is reproduced and explained.
Journal ArticleDOI
The Biochemical Properties of Manganese in Plants.
TL;DR: An overview of the chemistry and biochemistry of Mn in plants is presented, including an updated list of known Mn-dependent enzymes, together with enzymes where Mn has been shown to exchange with other metal ions.
Journal ArticleDOI
Energetics of proton release on the first oxidation step in the water-oxidizing enzyme
TL;DR: The present study suggests that the O4-water chain could facilitate the initial deprotonation event in PSII, and is likely to be of real relevance to mechanistic models for water oxidation.
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