Journal ArticleDOI
Cyclin activation of p34cdc2
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TLDR
The concerted transition into mitosis involves both a reduction in the rate of p34cdc2 phosphorylation on tyrosine and an increase in its rate of dephosphorylation.About:
This article is published in Cell.The article was published on 1990-11-30. It has received 607 citations till now. The article focuses on the topics: Cyclin A2 & Cyclin A.read more
Citations
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The p21 Cdk-interacting protein Cip1 is a potent inhibitor of G1 cyclin-dependent kinases
TL;DR: In this article, an improved two-hybrid system was employed to isolate human genes encoding Cdk-interacting proteins (Cips) and found that CIP1 is a potent, tight-binding inhibitor of Cdks and can inhibit the phosphorylation of Rb by cyclin A-Cdk2.
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Principles of CDK regulation
TL;DR: The activity of cyclin-dependent kinases is controlled by four highly conserved biochemical mechanisms, forming a web of regulatory pathways unmatched in its elegance and intricacy.
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Cyclin is degraded by the ubiquitin pathway
TL;DR: Cyclin degradation is the key step governing exit from mitosis and progress into the next cell cycle, and anaphase may be triggered by the recognition of cyclin by the ubiquitin-conjugating system.
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Colony-stimulating factor 1 regulates novel cyclins during the G1 phase of the cell cycle
Hitoshl Matsushime,Martine F. Roussel,Martine F. Roussel,Richard A. Ashmun,Charles J. Sherr,Charles J. Sherr +5 more
TL;DR: The timing of p36CYL expression, its rapid turnover in the absence of CSF-1, and its phosphorylation and transient binding to a cdc2-related polypeptide suggest that CYL genes may function during S phase commitment.
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Self-perpetuating states in signal transduction: positive feedback, double-negative feedback and bistability.
TL;DR: The basic properties of bistable circuits, the requirements for construction of a satisfactorybistable switch, and the recent progress towards constructing and analysing bistables signaling systems are reviewed.
References
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Journal ArticleDOI
Single-step purification of polypeptides expressed in Escherichia coli as fusions with glutathione S-transferase.
D B Smith,Kevin S. Johnson +1 more
TL;DR: Plasmid expression vectors have been constructed that direct the synthesis of foreign polypeptides in Escherichia coli as fusions with the C terminus of Sj26, a 26-kDa glutathione S-transferase (GST; EC 2.5.1.18) encoded by the parasitic helminth Schistosoma japonicum.
Journal ArticleDOI
Universal control mechanism regulating onset of M-phase
TL;DR: The onset of M-phase is regulated by a mechanism common to all eukaryotic cells and requires p34cdc2 dephosphorylation and association with cyclin.
Journal ArticleDOI
Cyclin: A protein specified by maternal mRNA in sea urchin eggs that is destroyed at each cleavage division
TL;DR: Eggs of the sea urchin Lytechinus pictus and oocytes of the surf clam Spisula solidissima also contain proteins that only start to be made after fertilization and are destroyed at certain points in the cell division cycle, and it is proposed to call these proteins the cyclins.
Journal ArticleDOI
Cyclin synthesis drives the early embryonic cell cycle.
TL;DR: In this paper, the addition of exogenous cyclin mRNA is sufficient to produce multiple cell cycles and the newly synthesized cyclin protein accumulates during each interphase and is degraded at the end of each mitosis.
Journal ArticleDOI
Tyrosine phosphorylation of the fission yeast cdc2 + protein kinase regulates entry into mitosis
Kathleen L. Gould,Paul Nurse +1 more
TL;DR: The cdc2+ protein kinase (pp34) is found to be phosphorylated on tyrosine as well as serine and threonine residues in exponentially growing Schizosaccharomyces pombe, establishing that tyrosines phosphorylation/dephosphorylation directly regulates pp34 function.
Related Papers (5)
Tyrosine phosphorylation of the fission yeast cdc2 + protein kinase regulates entry into mitosis
Kathleen L. Gould,Paul Nurse +1 more