Deoxyribonucleic Acid-dependent Ribonucleic Acid Polymerase Activity in Cells Infected with Influenza Virus
R. Borland,B. W. J. Mahy +1 more
TLDR
It is possible that host cell DNA-dependent RNA polymerase is involved in the replication of influenza virus RNA.Abstract:
Deoxyribonucleic acid (DNA)-dependent ribonucleic acid (RNA) polymerase activity was assayed on nuclear preparations of chick embryo fibroblast cells at various times after infection with an influenza A virus (fowl plague virus) and was compared with the activity of uninfected cells. Polymerase activity was increased by about 60% by 2 hr after infection, and this increase coincided with an increase in RNA synthesis in infected cells, as determined by pulse-labeling with uridine. No difference could be detected between the polymerases of infected and uninfected cells as to their requirements for DNA primer, divalent cations, and nucleoside triphosphates, and they were equally sensitive to addition of actinomycin D to the reaction mixture. It is possible that host cell DNA-dependent RNA polymerase is involved in the replication of influenza virus RNA.read more
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The synthesis of Sendai virus polypeptides in infected cells
Robert A. Lamb,Robert A. Lamb,Brian W. J. Mahy,Brian W. J. Mahy,Purnell W. Choppin,Purnell W. Choppin +5 more
TL;DR: The results of pulse and pulse-chase experiments suggest that large, polyprotein precursors are not involved in Sendai virus replication, and that polypeptides are synthesized from monocistronic messenger RNA species.
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Evidence for a ninth influenza viral polypeptide
TL;DR: The available evidence suggests that the synthesis of polypeptide 4 requires “early” protein synthesis, which is distinct from the eight defined influenza virus gene products.
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Polypeptides specified by the influenza virus genome: I. Evidence for eight distinct gene products specified by fowl plague virus
TL;DR: The structural polypeptides of fowl plague virus (influenza A) and those synthesized in fowl Plague virus-infected chick embryo fibroblasts have been analyzed by high resolution polyacrylamide gel electrophoresis and shown to be distinct by tryptic peptide mapping.
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Synthesis of influenza virus proteins in infected cells: Translation of viral polypeptides, including three P polypeptides, from RNA produced by primary transcription
TL;DR: Experiments in which cycloheximide was added at the time of infection to restrict viral RNA synthesis to primary transcription for various times after infection and then protein synthesis examined after removal of the drug have indicated that all of the viral polypeptides can be translated from mRNA produced by primary transcription.
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The smallest genome RNA segment of influenza virus contains two genes that may overlap
TL;DR: The genetic information for NS2 was found to reside in the smallest genome RNA segment of the virion, which also encodes the NS1 polypeptide, likely that the coding sequences for the twopolypeptides overlap.
References
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Journal Article
Protein Measurement with the Folin Phenol Reagent
TL;DR: Procedures are described for measuring protein in solution or after precipitation with acids or other agents, and for the determination of as little as 0.2 gamma of protein.
Journal ArticleDOI
Determination of serum proteins by means of the biuret reaction.
TL;DR: An investigation of the biochemical changes following experimental liver injury felt the need of a simple, rapid, and accurate method for determining the protein fractions in small amounts of serum and began with Kingsley’s biuret procedure.
Journal ArticleDOI
Action of actinomycin don animal cells and viruse
Journal ArticleDOI
Defective T particles of vesicular stomatitis virus. I. Preparation, morphology, and some biologic properties.
TL;DR: The data strongly suggest that T is a distinct, truncated form of B which contains only a portion of the VSV genome, and is the physical equivalent of the transmissible interfering component of Cooper and Bellett (1959).
Journal ArticleDOI
The effects of actinomycin d on growth of rous sarcoma virus in vitro
TL;DR: Low concentrations of actinomycin D (0.1 μg/ml) reversibly inhibit production of Rous Sarcoma virus (RSV) by Rous sarcoma cells and infection of cells and initiation of virus production are inhibited.