Journal ArticleDOI
Detection of erythrocytes influenced by aging and type 2 diabetes using atomic force microscope
Hua Jin,Xiaobo Xing,Hongxia Zhao,Hongxia Zhao,Yong Chen,Xun Huang,Shuyuan Ma,Hongyan Ye,Jiye Cai +8 more
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TLDR
The aging- and diabetes-induced changes in morphological and biomechanical properties of erythrocytes are clearly characterized at nanometer scale using atomic force microscope (AFM) to potentially be developed into a powerful tool in diagnosing diseases.About:
This article is published in Biochemical and Biophysical Research Communications.The article was published on 2010-01-22. It has received 74 citations till now. The article focuses on the topics: Insulin resistance & Type 2 diabetes.read more
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Investigating cell mechanics with atomic force microscopy.
Kristina Haase,Andrew E. Pelling +1 more
TL;DR: This review generally describes the mechanotransductive process through discussion of well-known mechanosensors, and focuses on discussion of recent examples where AFM is used to specifically probe the elastic and inelastic responses of single cells undergoing deformation.
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Changes in red blood cell membrane structure in type 2 diabetes: a scanning electron and atomic force microscopy study
Antoinette V. Buys,Mia-Jean Van Rooy,Prashilla Soma,Dirk van Papendorp,Boguslaw Lipinski,Etheresia Pretorius +5 more
TL;DR: It is shown that the combined AFM and SEM analyses of RBCs give valuable information about the disease status of patients with diabetes, as this cell’s health status is crucial to the overall wellness of the diabetic patient.
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A beginner's guide to atomic force microscopy probing for cell mechanics
TL;DR: This review is intended for those about to embark in the use of bio‐AFMs, and aims to assist them in designing an experiment to measure the mechanical properties of adherent cells.
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Automated force volume image processing for biological samples.
TL;DR: Two algorithms are proposed, one for the processing of approach force curves and another for the quantitative analysis of retraction force curves, to provide fully automated tools to achieve theoretical interpretation of force curves on the basis of adequate, available physical models.
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On the determination of elastic moduli of cells by AFM based indentation
TL;DR: A novel method to more accurately measure the mechanical properties of biological cells and soft materials in AFM indentation experiments is provided by using the neo-Hookean model to describe the hyperelastic behavior of cells and investigating the influence of surface tension through finite element simulations.
References
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Journal ArticleDOI
Nanomechanical analysis of cells from cancer patients
TL;DR: This work reports the stiffness of live metastatic cancer cells taken from the body fluids of patients with suspected lung, breast and pancreas cancer, and shows that nanomechanical analysis correlates well with immunohistochemical testing currently used for detecting cancer.
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Protofibrils, pores, fibrils, and neurodegeneration: separating the responsible protein aggregates from the innocent bystanders.
Byron Caughey,Peter T. Lansbury +1 more
TL;DR: Recent biophysical studies aimed at elucidating the precise mechanism of in vitro aggregation and animal modeling studies support the emerging notion that an ordered prefibrillar oligomer, or protofibril, may be responsible for cell death and that the fibril form that is typically observed at autopsy may actually be neuroprotective.
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Early detection of aging cartilage and osteoarthritis in mice and patient samples using atomic force microscopy
Martin Stolz,Riccardo Gottardi,Roberto Raiteri,Sylvie Miot,Ivan Martin,R. Imer,Urs Staufer,Aurelia Raducanu,Marcel Düggelin,Werner Baschong,Alma U. Daniels,Niklaus F. Friederich,Attila Aszódi,Ueli Aebi +13 more
TL;DR: It is shown that indentation-type atomic force microscopy can monitor age-related morphological and biomechanical changes in the hips of normal and osteoarthritic mice and may potentially be developed into a minimally invasive arthroscopic tool to diagnose the early onset of osteoartritis in situ.
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Local Nanomechanical Motion of the Cell Wall of Saccharomyces cerevisiae
Andrew E. Pelling,Sadaf Sehati,Edith Butler Gralla,Joan Selverstone Valentine,James K. Gimzewski +4 more
TL;DR: It is demonstrated that the cell wall of living Saccharomyces cerevisiae (baker's yeast) exhibits local temperature-dependent nanomechanical motion at characteristic frequencies, which is consistent with the cell's metabolism involving molecular motors such as kinesin, dynein, and myosin.
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Anti-microbial photodynamic therapy: useful in the future?
TL;DR: This chapter will focus on some aspects of anti-microbial photodynamic therapy that appear to be promising for dermatological indications and inactivation of pathogenic bacteria within the oral cavity.