Journal ArticleDOI
Development of an in vitro blood-brain barrier model-cytotoxicity of mercury and aluminum.
TLDR
In conclusion, BBB models composed of RBE4 and ARPE-19 cells were able to distinguish between different toxicities, and AR PE- 19 cells are thus promising candidates for studies of drug penetration through the blood-brain barrier.About:
This article is published in Toxicology and Applied Pharmacology.The article was published on 2004-02-15. It has received 58 citations till now. The article focuses on the topics: Confluency & Cell culture.read more
Citations
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Journal ArticleDOI
In Vitro Blood–Brain Barrier Models: Current and Perspective Technologies
Pooja Naik,Luca Cucullo +1 more
TL;DR: A detailed review and analysis of currently available in vitro BBB models ranging from static culture systems to the most advanced flow-based and three-dimensional coculture apparatus is provided.
Journal ArticleDOI
Prediction of Blood-Brain Barrier Permeation in Drug Discovery From in Vivo, in Vitro and in Silico Models
TL;DR: Higher throughout methods (cell- and non-cell-based; in silico modelling) can help in prediction of permeation, but a combination of techniques is still required to cover the range of BBB drug entry and efflux mechanisms.
Journal ArticleDOI
Cell-Culture Models of the Blood–Brain Barrier
TL;DR: This work has shown that restoring BBB integrity in pathological conditions to maintain brain homeostasis and opening BBB temporarily to allow efficient delivery of drugs to the CNS are potential therapeutic options for patients with these disorders.
Journal ArticleDOI
In-vitro blood-brain barrier models for drug screening and permeation studies: an overview
TL;DR: Although the complexity of the BBB (dynamic and adaptable barrier) affects approaches of CNS drug delivery and promotes disease progression, understanding the composition and functions of BBB provides a platform for novel innovative approaches towards drug delivery to CNS.
Journal ArticleDOI
Bovine and porcine transscleral solute transport: influence of lipophilicity and the Choroid-Bruch's layer.
TL;DR: The choroid-Bruch's layer is a more significant barrier to drug transport than is sclera and hinders the transport of lipophilic solutes, especially a cationic solute, more than hydrophilic solute and in a more dramatic way than does sClera.
References
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Journal ArticleDOI
Astrocytes induce blood–brain barrier properties in endothelial cells
Robert C. Janzer,Martin Raff +1 more
TL;DR: Direct evidence is provided that astrocytes are capable of inducing blood–brain barrier properties in non-neural endothelial cells in vivo.
Journal ArticleDOI
ARPE-19, A Human Retinal Pigment Epithelial Cell Line with Differentiated Properties
TL;DR: It was found that ARPE-19 cells exhibit morphological polarization when plated on laminin-coated Transwell-COL filters in medium with a low serum content and has structural and functional properties characteristic of RPE cells in vivo, suggesting that this cell line will be valuable for in vitro studies of retinal pigment epithelium physiology.
Journal ArticleDOI
A cell culture model of the blood-brain barrier.
Lee L. Rubin,D. E. Hall,S Porter,K Barbu,C. Cannon,H C Horner,M Janatpour,C. W. Liaw,K Manning,J Morales +9 more
TL;DR: A cell culture model of the blood-brain barrier is established by treating brain endothelial cells with a combination of astrocyte-conditioned medium and agents that elevate intracellular cAMP, potentially providing the basis for increasing the penetration of drugs into the central nervous system.
Journal ArticleDOI
Development of the blood-brain barrier.
Werner Risau,Hartwig Wolburg +1 more
TL;DR: Astrocytes that tightly appose endfeet onto the abluminal side of brain capillaries seem to be important for the induction and maintenance of the endothelial barrier.
Journal ArticleDOI
Blood-brain barrier biology and methodology
TL;DR: The classical physiologic techniques may now be correlated with modern biochemical and molecular biological approaches using freshly isolated animal or human brain capillaries to form an 'in vitro BBB' model.