Journal ArticleDOI
Development of L-histidine immobilized CIM(®) monolithic disks for purification of immunoglobulin G.
TLDR
The results indicate the high potential of this method for purification of total IgG from complex biological sources and also for separation of IgG1 from other subclasses.Abstract:
The pseudobiospecific affinity ligand l-histidine was immobilized on epoxy, carbonyldiimidazole (CDI), and ethylenediamine (EDA) convective interaction media (BIA Separations, Slovenia) monolithic disks to obtain the histidyl affinity column for purification of immunoglobulin G (IgG) The kinetics and the mass transfer properties of the affinity columns were studied to determine the optimum buffer condition, flow rate, and concentration of IgG for maximum IgG adsorption The binding capacities of all the three affinity columns were higher with zwitterionic buffer morpholinopropanesulfonic acid than with charged buffers such as tris-HCl and phosphate buffers, and the optimum pH was 65 The interaction of IgG with histidine immobilized CDI and epoxy disks was found to be predominantly driven by ionic interaction, while the interaction with EDA-histidine disk could be partially governed by multiple non-covalent forces of interaction The maximum binding capacity (Qm ) of l-histidine immobilized on EDA-, CDI-, and epoxy-activated convective interaction media disks were 1983 ± 025, 1585 ± 018 and 1211 ± 017 mg/ml of support, respectively, and the dissociation constant (Kd ) were calculated to be in the micromolar range for all the three histidyl monolithic columns Purification of IgG from untreated human serum was also attempted, and the results indicate the high potential of this method for purification of total IgG from complex biological sources and also for separation of IgG1 from other subclassesread more
Citations
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Journal ArticleDOI
Affinity chromatography: A versatile technique for antibody purification.
TL;DR: A detailed overview on affinity chromatography and the components involved in purification is provided to provide a detailed overview of antibody purification methodologies and their underlying working principles.
Journal ArticleDOI
Alternative Affinity Ligands for Immunoglobulins
Nika Kruljec,Tomaž Bratkovič +1 more
TL;DR: The design, development, and properties of diverse classes of alternative antibody-binding ligands, ranging from engineered versions of Ig-binding proteins, to artificial binding proteins, peptides, aptamers, and synthetic small-molecular-weight compounds are reviewed.
Journal ArticleDOI
Polydopamine meets porous membrane: A versatile platform for facile preparation of membrane adsorbers
TL;DR: The present work indicated that the polydopamine layer not only activated membrane surface to attach various adsorptive ligands under the mild condition, but also reduced non-specific adsorption.
Journal ArticleDOI
Affinity monolith chromatography: A review of general principles and recent developments
TL;DR: Affinity monolith chromatography (AMC) is a liquid chromatographic technique that utilizes a monolithic support with a biological ligand or related binding agent to isolate, enrich, or detect a target analyte in a complex matrix.
Journal ArticleDOI
Alginate dialdehyde meets nylon membrane: a versatile platform for facile and green fabrication of membrane adsorbers
TL;DR: The present work indicated that the ADA layer not only activated the membrane surface to attach various adsorptive ligands under mild conditions, but also reduced non-specific adsorption.
References
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Journal ArticleDOI
Metal chelate affinity chromatography, a new approach to protein fractionation
TL;DR: A highly flexible method based on affinities which can be used in a more selective fashion by modern chromatographic techniques is described here.
Journal ArticleDOI
Intravenous Immunoglobulin in Autoimmune Neuromuscular Diseases
TL;DR: Intravenous immunoglobulin is effective in many autoimmune neurologic diseases, but its spectrum of efficacy, especially as first-line therapy, and the appropriate dose for long-term maintenance therapy are not fully established.
Journal ArticleDOI
Purification of antibodies by affinity chromatography.
TL;DR: The purification strategies for antibodies by interaction with affinity ligands such as antibodies and Fe receptors or low molecular weight compounds are described.
Journal ArticleDOI
Pseudobiospecific ligand affinity chromatography
TL;DR: The mechanisms of the interactions between proteins and immobilized metals, dyes or histidine can be made specific enough to enable single-step purifications from biological mixtures are examined.
Journal ArticleDOI
Adsorption and elution of bovine γ-globulin using an affinity membrane containing hydrophobic amino acids as ligands
TL;DR: The reproducible profile of the flux and the protein concentration assured a quantitative cycle of chromatography using the affinity membrane containing Trp as a ligand, and a satisfactory quantitative elution was attained.