Different Mode of Action of Ribonuclease H in Purified α and αβ Ribonucleic Acid-directed Deoxyribonucleic Acid Polymerase from Avian Myeloblastosis Virus
TLDR
The β subunit of αβ may facilitate tighter binding of the enzyme to the substrate resulting in a processive mode of nuclease activity, which is supported by two types of experiments.About:
This article is published in Journal of Biological Chemistry.The article was published on 1974-08-25 and is currently open access. It has received 55 citations till now. The article focuses on the topics: Exoribonuclease activity & RNase H.read more
Citations
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Isothermal, in vitro amplification of nucleic acids by a multienzyme reaction modeled after retroviral replication.
John C. Guatelli,Kristina Marie Whitfield,Deborah Y. Kwoh,Kevin J. Barringer,Douglas D. Richman,Thomas R. Gingeras +5 more
TL;DR: A target nucleic acid sequence can be replicated exponentially in vitro under isothermal conditions by using three enzymatic activities essential to retroviral replication: reverse transcriptase, RNase H, and a DNA-dependent RNA polymerase, and this reaction accumulates cDNA and RNA copies of the original target.
Patent
Nucleic acid sequence amplification methods
TL;DR: In this paper, a method for synthesizing multiple copies of a target nucleic acid sequence autocatalytically under conditions of substantially constant temperature, ionic strength, and pH is presented.
Journal ArticleDOI
Human immunodeficiency virus 1 reverse transcriptase. Template binding, processivity, strand displacement synthesis, and template switching.
TL;DR: The kinetics of DNA synthesis catalyzed by reverse transcriptase from human immunodeficiency virus 1 (HIV-1) is analyzed, finding processivity varies widely with the template used, increasing from a few to greater than 300 nucleotides in the order.
Journal ArticleDOI
Polymerization and RNase H activities of the reverse transcriptases from avian myeloblastosis, human immunodeficiency, and Moloney murine leukemia viruses are functionally uncoupled.
Jeffrey J. DeStefano,R G Buiser,L M Mallaber,Thomas W. Myers,Robert A. Bambara,Philip J. Fay +5 more
TL;DR: Results demonstrate that the RNase H function is much less active than the polymerization function during processive DNA synthesis and that the activities are not strictly coupled.
References
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Journal ArticleDOI
A new method for the large scale purification of Escherichia coli deoxyribonucleic acid-dependent ribonucleic acid polymerase.
TL;DR: A method for the purification of Escherichia coli DNA-dependent RNA polymerase which is rapid, reproducible, high in yield, and able to handle preparations using from 1 g to 3 kg of cells is described.
Journal ArticleDOI
RNA Synthesis Initiates In Vitro Conversion of M13 DNA to Its Replicative Form
TL;DR: Rifampicin does not block synthesis of the replicative forms of varphiX174 either in vivo or in soluble extracts, and DNA synthesis with deoxyribonucleoside triphosphates and a fresh addition of enzymes is not required.
Journal ArticleDOI
Enzyme from Calf Thymus Degrading the RNA Moiety of DNA-RNA Hybrids: Effect on DNA-Dependent RNA Polymerase
Hans Stein,Peter Hausen +1 more
TL;DR: If prepared free of the hybrid-degrading enzyme, RNA polymerase from calf thymus shows a fivefold increase in activity on denatured DNA as compared to native DNA.
Journal ArticleDOI
Association of viral reverse transcriptase with an enzyme degrading the RNA moiety of RNA-DNA hybrids.
TL;DR: This work has demonstrated that RNA-DNA hybrids have been demonstrated to occur as intermediates in this reaction and single stranded DNA is generated as an early reaction product, which is then replicated to give a double stranded DNA product.
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Degradation of DNA RNA Hybrids by Ribonuclease H and DNA Polymerases of Cellular and Viral Origin
Walter Keller,Robert J. Crouch +1 more