Journal ArticleDOI
Differential antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites to chromium stress in green gram ( Vigna radiata L. wilczek) leaves
Thirupathi Karuppanapandian,Pritam Bala Sinha,Abdul Majeeth Kamarul Haniya,Kumariah Mamoharan +3 more
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It is demonstrated that Cr-induced oxidative stress is an important component of the plant’s reaction to toxic levels of Cr and that increased enzyme activities would be responsible for the removal of H2O2.Abstract:
Chromium-induced antioxidative responses of ascorbate-glutathione cycle enzymes and metabolites in green gram(Vigna radiata L. Wilczek) leaves were investigated in both dose and time-dependent manners. Rapid uptake of Cr was observed immediately after the start of treatment. Significant reduction was observed in leaf biomass under 300 µM Cr-treatment. Treatment with 300 µM Cr increases the content of hydrogen peroxide and Superoxide dismytase activity upto initial 96 h, and then gradually declined to the basal level. Ascorbate peroxidase and guaiacol peroxidase activities were low in 300 µM Cr-treated leaves during the first 96 h, but significantly increased therefore, suggesting that increased enzyme activities would be responsible for the removal of H2O2. Catalase activities were always suppressed under Cr stress. Contents of reduced ascorbate and dehydroascorbate were significantly decreased under 300 uM Cr-treatment. The reduced glutathione content decreased at early stages of Cr-treatment. However, it was restored to the normal level as in controls thereafter. In contrast, the glutathione disulphide content showed a progressive increase during the initial hours of Cr-treatment. The non-protein thiol content was shown to increase during the first several hours, but it declines at later stages. The present results demonstrate that Cr-induced oxidative stress is an important component of the plant’s reaction to toxic levels of Cr.read more
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Uncommon heavy metals, metalloids and their plant toxicity: a review
TL;DR: In this article, the phytotoxicity of rare heavy metals and metalloids such as tellurium, germanium, gallium, scandium, gold, platinum group metals (palladium, platinum and rhodium), technetium, tungsten, uranium, thorium, and rare earth elements yttrium and lanthanum are reviewed.
Journal ArticleDOI
2,4-dichlorophenoxyacetic acid-induced leaf senescence in mung bean (Vigna radiata L. Wilczek) and senescence inhibition by co-treatment with silver nanoparticles.
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TL;DR: It is suggested that increased oxidative stress and H(2)O(2)) led to senescence in mung bean leaves and significantly induced antioxidative enzymes are not sufficient to protect mungbean cells from 2,4-D-induced harmful ROS.
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Drought Stress Tolerance in Plants, Vol 1
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References
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Tissue sulfhydryl groups
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TL;DR: In this article, the catalytic activity of catalase has been investigated using ultraviolet (UV) spectrophotometry and Titrimetric methods, which is suitable for comparative studies for large series of measurements.
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Superoxide dismutase: Improved assays and an assay applicable to acrylamide gels☆
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