Journal ArticleDOI
Divalent cation induced changes in structural properties of the dimeric enzyme glucose oxidase: dual effect of dimer stabilization and dissociation with loss of cooperative interactions in enzyme monomer.
TLDR
The effect of the divalent cations Ca2+ and Mg2+ on the structural and stability properties of GOD was reported and it was observed that the 3 M CaCl2-stabilized monomer of GOD retained about 70% secondary structure present in the native GOD dimer; however, there was a complete loss of cooperative interactions between these secondary structural elements present inThe enzyme.Abstract:
Glucose oxidase (GOD) from Aspergillus niger is a dimeric enzyme having high localization of negative charges on the enzyme surface and at the dimer interface. The monovalent cations induce compact...read more
Citations
More filters
Journal ArticleDOI
Irreversible Thermal Denaturation of Glucose Oxidase from Aspergillus niger Is the Transition to the Denatured State with Residual Structure
TL;DR: The transition temperature is independent of the protein concentration, and the properties of the thermally Denatured protein indicate that thermally denatured GOX is a compact structure, a form of molten globule-like apoenzyme.
Journal ArticleDOI
Engineering glucose oxidase for bioelectrochemical applications.
TL;DR: The recent progress in glucose oxidase reengineering is presented and the need to develop new screening methods involving electrochemical probing, essential to develop the next generation of glucose sensors; specific to glucose, O2 independent, biocompatible and stable over 2 weeks is highlighted.
Patent
Wound dressings comprising hydrated hydrogels and enzymes
TL;DR: A skin dressing comprises a hydrated hydrogel material including a source of lactate ions and a supply of glucose, optionally also including an additional source of zinc ions and iodide ions as mentioned in this paper.
Journal ArticleDOI
Electrochemical activity of glucose oxidase on a poly(ionic liquid) - Au nanoparticle composite.
TL;DR: Glucose oxidase (GOx) adsorbed on an ionic liquid-derived polymer containing internally organized columns of Au nanoparticles exhibits direct electron transfer and bioelectrocatalytic properties towards the oxidation of glucose, allowing it to function as an electrode for electrochemical detection.
Journal ArticleDOI
A new insight into electrochemical microRNA detection: a molecular caliper, p19 protein.
TL;DR: For the first time an electrochemical biosensor for detection of mir21 using the oxidation signal of protein 19 (p19) as a molecular caliper was designed and the proposed method enables detection of miRNAs in direct, rapid, sensitive, inexpensive and label-free way.
References
More filters
Journal ArticleDOI
Crystal structure of glucose oxidase from Aspergillus niger refined at 2.3 A resolution.
TL;DR: The crystal structure of the partially deglycosylated enzyme from Aspergillus niger has been determined by isomorphous replacement and refined to 2.3 A resolution and the substrate-binding domain is topologically very similar to other FAD-binding proteins.
Journal ArticleDOI
Purification, properties, and molecular features of glucose oxidase from Aspergillus niger.
TL;DR: FHD (flavin-hypoxanthine dinucleotide) has coenzymatic activity equal to that of FAD (FAD) and could be a new treatment for central nervous system disorders according to EMT results reported in EMT4.4.
Journal ArticleDOI
Molecular relaxation spectroscopy of flavin adenine dinucleotide in wild type and mutant lipoamide dehydrogenase from Azotobacter vinelandii.
Philippe I. H. Bastiaens,Arie van Hoek,Willem J. H. van Berkel,Aart de Kok,Antonie J. W. G. Visser +4 more
TL;DR: It can be concluded that the C-terminal polypeptide of the A. vinelandii enzyme interacts with the dehydrolipoamide binding site, thereby shielding the flavins from the solvent.
Journal ArticleDOI
Förster energy transfer from tryptophan to flavin in glucose oxidase enzyme
TL;DR: The atomic absorption of a glucose oxidase solution shows that the enzyme is not associated to any metals which could be responsible for the tryptophan fluorescence quenching effect and that the strength of this coupling is dependent on the flavin redox state.