DNA-RNA hybrid duplexes containing oligo(dA:rU) sequences are exceptionally unstable and may facilitate termination of transcription.
Francis H. Martin,Ignacio Tinoco +1 more
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TLDR
This work has shown that a DNA-RNA hybrid oligonucleotide duplex, dC(pA) 5pG:rC( pU)5pG, which contains a (dA:rU) 5 sequence, is at least 200 times less stable at room temperature than the corresponding duplex containing an (rA:dT) 5 sequences.Abstract:
A DNA-RNA hybrid oligonucleotide duplex, dC(pA) 5pG:rC(pU)5pG, which contains a (dA:rU) 5 sequence, is at least 200 times less stable at room temperature than the corresponding duplex containing an (rA:dT) 5 sequence, rC(pA)5pG:dC(pT)5pG. This result provides an explanation for the finding that most primary RNA transcripts terminate in several consecutive rU residues, but not rA residues. It strongly supports the idea that instability of the DNA-RNA hybrid at the growing point of transcription plays a role in termination of transcription.read more
Citations
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Predicting DNA duplex stability from the base sequence.
TL;DR: This work reports the complete thermodynamic library of all 10 Watson-Crick DNA nearest-neighbor interactions and shows how these thermodynamic data can be used to calculate the stability and predict the temperature-dependent behavior of any DNA duplex structure from knowledge of its base sequence.
Journal ArticleDOI
Calculating thermodynamic data for transitions of any molecularity from equilibrium melting curves
TL;DR: The general equations and methods of analysis described in this paper should be of particular interest to laboratories that currently are investigating association and dissociation processes in nucleic acids that exhibit molecularities greater than two.
Journal ArticleDOI
Thermodynamic Parameters To Predict Stability of RNA/DNA Hybrid Duplexes
Naoki Sugimoto,Shu-ichi Nakano,Misa Katoh,Akiko Matsumura,Hiroyuki Nakamuta,Tatsuo Ohmichi,Mari Yoneyama,Muneo Sasaki +7 more
TL;DR: The thermodynamic parameters (delta H degree, delta S degree, and delta G degree 37) for 16 nearest-neighbor sets and one initiation factor are presented here in order to predict stability of RNA/DNA hybrid duplexes.
Journal ArticleDOI
Synthesis and hybridization studies on two complementary nona(2'-O-methyl)ribonucleotides.
TL;DR: It was found that the 2'-O-methyl oligoribonucleotides can be utilized as an alternative to an oligonucleotide probe in RNA hybridizations as the hybrid formed has a high, or a higher Tm, the probe is much easier to synthesize and it is less likely to be enzymatically degraded.
Journal ArticleDOI
Nucleotide sequences in Xenopus 5S DNA required for transcription termination
TL;DR: In this paper, the DNA sequences required for termination of Xenopus 5S RNA synthesis in vitro were analyzed and it was shown that termination occurs within clusters of four or more consecutive T residues in the noncoding DNA strand sequence.
References
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Journal ArticleDOI
Regulatory Sequences Involved in the Promotion and Termination of RNA Transcription
Martin Rosenberg,Donald L. Court +1 more
Journal ArticleDOI
Control of Transcription Termination
Sankar Adhya,Max E. Gottesman +1 more
TL;DR: The author revealed that RNA polymerase mutations located at the Site of Termination Site are linked to RHO mutations, and that pN acts at Many Sites and recognition Specificity of pN is recognized.
Journal ArticleDOI
Physical and chemical characterization of two- and three-stranded adenine-thymine and adenine-uracil homopolymer complexes.
Monica Riley,Barbara Maling +1 more
TL;DR: The DNA homopolymers dA and dT have been prepared enzymically using Escherichia coli DNA polymerase, and their properties have been studied, but the homopolymer pair dA:rU is not stable under any conditions of temperature and salt concentration tested.
Journal ArticleDOI
Structures for the polynucleotide complexes poly(dA) · poly(dT) and poly(dT) · poly(dA) · poly(dT)
Struther Arnott,Erik Selsing +1 more
TL;DR: X-ray diffraction analyses of fibers of polydeoxyadenylic acid · polydeoxythymidylic acid show that this molecule exists as a 10-fold double-helix with axial rise per nucleotide h = 3.24 to 3.29 A.
Journal ArticleDOI
R loop mapping of the 18S and 28S sequences in the long and short repeating units of drosophila melanogaster rDNA
TL;DR: R loop mapping of a population of long units obtained directly from the rDNA has demonstrated that this interruption of the sequence coding for the 28S rRNA is a characteristic of the long class.
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