Journal ArticleDOI
DNA-templated silver nanoclusters for multiplexed fluorescent DNA detection.
Ying Zhang,Ying Zhang,Changfeng Zhu,Lei Zhang,Chaoliang Tan,Jian Yang,Bo Chen,Lianhui Wang,Hua Zhang +8 more
TLDR
A new method for multiple detection of biological molecules by using fluorescent Ag nanocluster-based molecular beacon probes using DNA templated-silver nanoclusters for multiplexed DNA detection is demonstrated.Abstract:
Novel label-free/conjugation-free molecular beacons are designed based on DNA templated-silver nanoclusters for multiplexed DNA detection. The assay is implemented in solution, which makes it easy for the in-situ and real-time analysis. This study demonstrates a new method for multiplexd detection of biological molecules by using fluorescent Ag nanocluster-based molecular beacon probes.read more
Citations
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Metal nanoclusters: novel probes for diagnostic and therapeutic applications
TL;DR: The rapidly growing interest in metal nanocluster-based theranostic applications will certainly not only fuel the excitement and stimulate research in this highly active field, but also inspire broader concerns across various disciplines.
Polymorphisms at Nucleotide Resolution with a Single DNA Microarray
David Gresham,Douglas M. Ruderfer,Stephen C. Pratt,Joseph Schacherer,Maitreya J. Dunham,David Botstein,Leonid Kruglyak +6 more
TL;DR: A system to detect all single-nucleotide differences between genomes with the use of data from a single hybridization to a whole-genome DNA microarray was devised, which allowed it to detect a variety of spontaneous single–base pair substitutions, insertions, and deletions.
Journal ArticleDOI
Nanotechnology-Enhanced No-Wash Biosensors for in Vitro Diagnostics of Cancer.
TL;DR: This review provides a comprehensive overview of various nanomaterial-enhanced no-wash biosensing technologies and focuses on the analysis of the underlying mechanism of these technologies applied for the early detection of cancer biomarkers ranging from small molecules to proteins, and even whole cancerous cells.
Journal ArticleDOI
DNA metallization: principles, methods, structures, and applications
TL;DR: This review presents a comprehensive review to summarize the research activities on DNA metallization that have appeared since the concept was first proposed in 1998, and discusses the applications of these metal nanomaterials in the fields mentioned above.
Journal ArticleDOI
Multiplexed photoluminescent sensors: towards improved disease diagnostics
TL;DR: A selection of recent contributions towards multiplexed photoluminescent sensors which can simultaneously detect multiple and diverse biomarkers that exist in a homogenous solution or a single cell, accelerating the progress towards precise disease diagnosis are highlighted.
References
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Journal ArticleDOI
Molecular Beacons: Probes that Fluoresce upon Hybridization
Sanjay Tyagi,Fred Russell Kramer +1 more
TL;DR: Novel nucleic acid probes that recognize and report the presence of specific nucleic acids in homogeneous solutions that undergo a spontaneous conforma-tional change when they hybridize to their targets are developed.
Journal ArticleDOI
Pathogen detection: a perspective of traditional methods and biosensors.
TL;DR: This review not only offers an overview of trends in the area of pathogen detection but it also describes main techniques, traditional methods, and recent developments in the field of pathogenic bacteria biosensors.
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Single-mismatch detection using gold-quenched fluorescent oligonucleotides.
TL;DR: It is shown that this composite molecule is a different type of molecular beacon with a sensitivity enhanced up to 100-fold and the ability to detect single mismatch is eightfold greater with this probe than with other molecular beacons.
Journal ArticleDOI
An overview of foodborne pathogen detection: In the perspective of biosensors
TL;DR: The conventional methods, analytical techniques and recent developments in food pathogen detection, identification and quantification, with an emphasis on biosensors are described.
Journal ArticleDOI
DNA-templated Ag nanocluster formation.
TL;DR: Fluorescence, absorption, mass, and NMR spectra all indicate that multiple species are present, but that their creation is both nucleotide- and time-dependent, and that the nanoclusters are associated with the chiral ss-DNA template.