Domain interaction in rabbit muscle pyruvate kinase. II. Small angle neutron scattering and computer simulation.
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TLDR
The effects of ligands on the structure of rabbit muscle pyruvate kinase were studied by small angle neutron scattering, and results indicate a "contraction" and "expansion" of the enzyme when it transforms between its active and inactive forms.About:
This article is published in Journal of Biological Chemistry.The article was published on 1988-02-25 and is currently open access. It has received 35 citations till now. The article focuses on the topics: Small-angle neutron scattering & Neutron scattering.read more
Citations
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Journal ArticleDOI
Clostridium difficile toxins a and b are cation-dependent udp-glucose hydrolases with differing catalytic activities
William P. Ciesla,David A. Bobak +1 more
TL;DR: It is concluded that C. difficile toxins A and B are cation-dependent UDP-glucose hydrolases that differ significantly in their catalytic activities, a finding that may have important implications in understanding their different cytotoxic effects.
Journal ArticleDOI
Allosteric Mechanism of Pyruvate Kinase from Leishmania mexicana Uses a Rock and Lock Model
Hugh P. Morgan,Iain W. McNae,Matthew W. Nowicki,Véronique Hannaert,Paul A.M. Michels,Linda A. Fothergill-Gilmore,Malcolm D. Walkinshaw +6 more
TL;DR: The results presented here illustrate how conformational changes coupled with effector binding correlate with loss of flexibility and increase in thermal stability providing a general mechanism for allosteric control.
Journal ArticleDOI
Structural and functional linkages between subunit interfaces in mammalian pyruvate kinase.
John O. Wooll,Robert H.E. Friesen,Mark A. White,Stanley J. Watowich,Robert O. Fox,James J. Lee,Edmund W. Czerwinski +6 more
TL;DR: The results identify the structural linkages between the Y and Z interfaces in regulating the interconversion of conformational states of rabbit M1-PK.
Journal ArticleDOI
Enhanced Diffusion of Catalytically Active Enzymes.
Yifei Zhang,Henry Hess +1 more
TL;DR: It is pointed out that the existing multimeric forms of enzymes or isozymes may cause artifacts in measurements and that the conformational changes upon substrate binding are usually not sufficient to give rise to a diffusion enhancement greater than 30% so more rigorous experiments and a more comprehensive theory are urgently needed to quantitatively validate and describe the enhanced enzyme diffusion.
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Global conformational changes in allosteric proteins. A study of Escherichia coli cAMP receptor protein and muscle pyruvate kinase.
TL;DR: For two systems, namely Escherichia coli cAMP receptor protein and muscle pyruvate kinase, the results show that negative and positive cooperativity are correlated with sequential and concerted modes of structural change, respectively.
References
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Book ChapterDOI
[66] Pyruvate kinase from muscle: Pyruvate phosphokinase, pyruvic phosphoferase, phosphopyruvate transphosphorylase, phosphate—transferring enzyme II, etc. Phosphoenolpyruvate + ADP ⇌ Pyruvate + ATP
Theodor Bücher,G. Pfleiderer +1 more
Journal ArticleDOI
Crystal structure of cat muscle pyruvate kinase at a resolution of 2.6 A
TL;DR: The structure of pyruvate kinase (EC 2.7.1.40) has been determined from a 2.6 A resolution electron density map and a detailed chain folding to be established for two out of the three domains which make up each of the four identical subunits.
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Small-angle scattering. Information content and error analysis
TL;DR: In this article, an analysis of the small-angle scattering problem based on Shannon sampling is presented, which leads to an accurate assessment of the information contained in a given data set and rigorous estimates of the errors inherent in the parameters derived therefrom.
Journal ArticleDOI
Glucose-induced conformational change in yeast hexokinase
TL;DR: It is found that the hydrophobic effect alone favors the active conformation of hexokinase in the presence and absence of sugar.