Draconibacterium orientale gen. nov., sp. nov., isolated from two distinct marine environments, and proposal of Draconibacteriaceae fam. nov.
TLDR
On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., within the class Bacteroidia, are proposed, with strain FH5T (=DSM 25947T=CICC 10585T) as the type strain.Abstract:
The taxonomic characteristics of two bacterial strains, FH5T and SS4, isolated from enrichment cultures obtained from two distinct marine environments, were determined. These bacteria were Gram-stain-negative, facultatively anaerobic rods. Growth occurred at 20–40 °C (optimum, 28–32 °C), pH 5.5–9.0 (optimum, pH 7.0–7.5) and in the presence of 1–7 % NaCl (optimum, 2–4 %). The major cellular fatty acids were anteiso-C15 : 0 and iso-C15 : 0. Menaquinone 7 (MK-7) was the sole respiratory quinone. The major polar lipids were phosphatidylethanolamine, an unkown phospholipid and an unknown lipid. The DNA G+C contents of strains FH5T and SS4 were both determined to be 42.0 mol%. The results of DNA–DNA hybridization studies indicated that the FH5T and SS4 genomes share greater than 95 % relatedness. The strains formed a distinct phyletic line within the class
Bacteroidia
, with less than 89.4 % sequence similarity to their closest relatives with validly published names. On the basis of physiological and biochemical characteristics, 16S rRNA gene sequences and chemical properties, a novel genus and species, Draconibacterium orientale gen. nov., sp. nov., within the class
Bacteroidia
, are proposed, with strain FH5T ( = DSM 25947T = CICC 10585T) as the type strain. In addition, a new family, Draconibacteriaceae fam. nov., is proposed to accommodate Draconibacterium gen. nov.read more
Citations
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Lentimicrobium saccharophilum gen. nov., sp. nov., a strictly anaerobic bacterium representing a new family in the phylum Bacteroidetes, and proposal of Lentimicrobiaceae fam. nov
Liwei Sun,Liwei Sun,Mayu Toyonaga,Akiko Ohashi,Dieter M. Tourlousse,Norihisa Matsuura,Xian-Ying Meng,Hideyuki Tamaki,Satoshi Hanada,Rodrigo Cruz,Takashi Yamaguchi,Yuji Sekiguchi +11 more
TL;DR: Based on its 16S rRNA gene sequence, strain TBC1T was shown to represent a distinct lineage at the family level in the phylum Bacteroidetes and is considered to be the type strain of a novel species of a new genus, Lentimicrobium saccharophilum gen. nov.
Journal ArticleDOI
Revised phylogeny of Bacteroidetes and proposal of sixteen new taxa and two new combinations including Rhodothermaeota phyl. nov.
TL;DR: A revision of an earlier phylogeny of Bacteroidetes has been performed using the 16S rRNA gene as a backbone in combination with the 23S r RNA gene, as well as multilocus sequence analysis (MLSA) of 29 orthologous protein sequences, and indels in the sequences of the beta subunit of the F-type ATPase and the alanyl-tRNA synthetase.
Species concept for prokaryotes
TL;DR: The validity of the concept in use is discussed, which is believed to be more pragmatic in comparison with those concepts described for eukaryotes.
Journal ArticleDOI
Metatranscriptomic and comparative genomic insights into resuscitation mechanisms during enrichment culturing.
TL;DR: An enrichment culture system for high-efficiency isolation of uncultured strains from marine sediment showed that the so-called enrichment method could culture the “uncultured” not only through enriching the abundance of ”uncultures” but also through the resuscitation mechanism.
Journal ArticleDOI
Tangfeifania diversioriginum gen. nov., sp. nov., a representative of the family Draconibacteriaceae.
TL;DR: A novel Gram-stain-negative, facultatively anaerobic, catalase- and oxidase-positive, non-motile and pink-pigmented bacterium, designated G22(T), was isolated from Gahai, a saltwater lake in Qinghai province, China.
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