Dynamic Imaging of Cerebral Blood Flow Using Laser Speckle
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TLDR
Using this method, dynamic images of the relative CBF changes during focal cerebral ischemia and cortical spreading depression were obtained along with electrophysiologic recordings and validated through direct comparison with conventional laser-Doppler measurements.Abstract:
A method for dynamic, high-resolution cerebral blood flow (CBF) imaging is presented in this article. By illuminating the cortex with laser light and imaging the resulting speckle pattern, relative CBF images with tens of microns spatial and millisecond temporal resolution are obtained. The regional CBF changes measured with the speckle technique are validated through direct comparison with conventional laser-Doppler measurements. Using this method, dynamic images of the relative CBF changes during focal cerebral ischemia and cortical spreading depression were obtained along with electrophysiologic recordings. Upon middle cerebral artery (MCA) occlusion, the speckle technique yielded high-resolution images of the residual CBF gradient encompassing the ischemic core, penumbra, oligemic, and normally perfused tissues over a 6 × 4 mm cortical area. Successive speckle images demonstrated a further decrease in residual CBF indicating an expansion of the ischemic zone with finely delineated borders. Dynamic CBF...read more
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References
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Thresholds in cerebral ischemia - the ischemic penumbra.
TL;DR: Recent evidence indicates that immediate failure of basic functions such as synaptic transmission, ion pumping and energy metabolism in the ischemic brain, is critically dependent on residual blood flow, and that these functions fail at certain critical flow thresholds.
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Spreading depression of activity in the cerebral cortex
TL;DR: In this article, an interesting response elicited by electrical stimulation was noticed in the cortex of rabbits, and the distinctive feature of this response was a marked, enduring reduction of the "spontaneous" electrical activity of the cortex.
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Viability thresholds and the penumbra of focal ischemia.
TL;DR: It is suggested that the limited survival of the penumbra is due to periinfarct depolarizations, which result in repeated episodes of tissue hypoxia, because the increased metabolic workload is not coupled to an adequate increase of collateral blood supply.
Journal ArticleDOI
Functional architecture of cortex revealed by optical imaging of intrinsic signals
Amiram Grinvald,Amiram Grinvald,Amiram Grinvald,Edmund E. Lieke,Ron D. Frostig,Charles D. Gilbert,Torsten N. Wiesel +6 more
TL;DR: It is shown that some intrinsic changes in the optical properties of the tissue are dependent on electrical or metabolic activity and can be used to study the functional architecture of cortex.
Journal ArticleDOI
Measurement of local cerebral blood flow with iodo [14C] antipyrine
TL;DR: Iodo[14C]antipyrine appears, therefore, to be a satisfactory nonvolatile tracer for the measurement of local cerebral blood flow.
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